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FDSS/μCELL 功能性药物筛查系统
C13299
FDSS/μCELL 是一个实验室筛选系统，它紧凑地集成了在药物发现筛选中开发的技术，实现了易于使用的专用系统。
通过高灵敏度二维传感器（相机）和分配器头（96 孔型/384 孔型），可以同时测量和分析在添加化合物时所有孔中样品的荧光或发光强度的动力学。通过在相同条件下进行测量，可以高通量筛选各种化合物，各孔之间没有时滞。
特点
- 适用于荧光/发光分析
- 在 96 孔/384 孔中同时添加和读取
- 使用各种波长的激发光源进行宽范围测量
- 长寿命、大功率 LED 激发光源
- 通过改变波长适用于 FRET 或 BRET
- 高速数据采集，最长 5 ms（可选）
- 同时电刺激和读取 96 孔（可选）
- 外部控制选件可用于自动化（可选）
- 通过安装加热器单元（可选），可将温度保持在 +35 ºC 至 +37 ºC
- CO2 培养（可选）
- 波形分析（可选）
用途
1. GPCR
GPCR（G 蛋白偶联受体）在细胞信号转导中起着重要作用，许多靶向 GPCR 医疗药物已开发出来。FDSS/μCELL 能够通过使用荧光和发光探针来检测信使（如 Ca2+ 和 cAMP），它们是 GPCR 信号转导系统的主要贡献者。FDSS/μCELL 允许在整个微孔板孔中同时分配和动力学测量化合物，从而实现高通量筛选。
・Ca2+ 测量：Fluo-4、Fluo-8、Cal-520、水母发光蛋白
・cAMP 测量：Glo-传感器
2. 离子通道
离子通道是一类跨膜蛋白，允许某些离子穿过细胞生物膜（进入或离开细胞），调节细胞功能，并参与心血管、神经系统和代谢疾病的发展。FDSS/μCELL 使用电压敏感荧光染料或不同离子的荧光指示剂进行高通量药物筛选。
・Na+ 测量：ANG-2、Corona-Green、Corona-Red、Sodium-Green
・K+ 测量：FluxOR
・Cl- 测量：MEQ、MQAE、YFP
・膜电位测量：FluoVolt、Di-8-ANEPPS、DiBAC4 (3)
3. 发光
使用发光探针进行检测的优点（即高灵敏度和低噪声），已经导致此类检测广泛应用于各种荧光素酶检测系统和使用水母蛋白的 Ca2+ 测定中。FDSS/μCELL 使用高度灵敏的二维传感器（相机）在单个微孔板上使用发光探针同时执行不同的检测，从而在添加底物后进行高通量筛选，而不会造成时间滞后。
4. BRET / FRET
使用 GFP（绿色荧光蛋白）或 Luc（荧光素酶）的共振能量转移原理的生物传感器被用作测量各种细胞内信号传输的工具，包括离子浓度和信号传导分子活性。FDSS/μCELL 使用高度灵敏的二维传感器（相机）和自动滤波器更换器，在单个微孔板上同时执行 BRET（生物发光共振能量转移）测量、基于发光的方法和 FRET（荧光共振能量转移）测量、基于荧光的方法。
・BRET：BRET1、BRET2、Nano-BRET
・FRET：C/Y FRET、VSP、Cameleon
5. 诱导性多能干细胞
最近已从 iPSC（诱导性多能干细胞）产生各种分化细胞，并且这日益支持使用人源天然细胞进行基于细胞的检测。特别是，iPS 心脏毒性、iPS 神经毒性和 iPS 肝毒性评估日益用作化合物的安全性评估。FDSS 执行高通量毒性筛选。
此外，可选的 EFS 头部起搏系统还支持使用心肌细胞、骨骼肌细胞和神经元的 FDSS 平台的全新用途。
详细参数
| 分配（96 针型）A10118-24 | 10 μL 至 200 μL |
|---|---|
| 分配（384 针型）A10118-26 | 1 μL 至 30 μL |
| 传感器 (ImagEM) | 用于荧光和发光的高速、高灵敏度数字 EM-CCD 相机 |
| 采样率 | 10 Hz（每秒 10 个数据点） 最大 200 Hz（每秒 200 个数据点），带 U8524-11 选件 |
| 采样间隔 | 0.1 s 至 100 s 间隔 0.005 s 至 100 s 间隔，带 U8524-11 选件 |
| 光源 (L11601-06) | 470 nm 激发和 540 nm 发光 530 nm 激发和 593 nm 发光 |
| 板位置 | 一个载物台用于测定板，两个载物台用于化合物板 |
| 适应性微孔板 | 透明底部黑色 96/384 板（SBS 格式高度 8 mm 至 15 mm） |
| 吸头/板加载 | 手动加载 |
| 采样数据点数量 | 1 至 50000 次采样 |
| 电源规格 | 输入电源：交流 100 V 至交流 240 V，频率：50 Hz/60 Hz |
| 交流 100 V 至交流 120 V 时的功耗 （数据分析单元和带加热器的 FDSS/μCELL 主机） | 约 1300 VA （数据分析单元：约 500 VA，分配器主机：约 300 VA，加热器单元：约 500 VA） |
| 交流 200 V 至交流 240 V 时的功耗 （数据分析单元和带加热器的 FDSS/μCELL 主机） | 约 1170 VA （数据分析单元：约 500 VA，分配器主机：约 300 VA，加热器单元：约 370 VA） |
| 环境工作温度 | +15°C 至 +30°C |
| 尺寸/重量 主机（不包括数据分析单元） | 550 mm (W)×675 mm (D)×1600 mm (H)，重约 200 kg |
尺寸
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