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酶测定 | 动态板成像仪
FDSS 是荧光和发光领域的“添加和读取”动态板成像仪，适用于读取那些反应、钙通量、流动、膜电位变化的活细胞的动力学，这种变化非常快，需要监测其动力学特性。GPCR 和离子通道以及转运蛋白是 FDSS 动态板成像仪最常用的应用领域，用于监测活细胞的钙瞬态和膜电位变化。
然而，在酶领域也做了一些创造性的工作，FDSS 现在并不限于活细胞。通常，酶测定不需要“添加和读取”动态板成像仪，因为其反应进行得非常缓慢，因此每 30 分钟或每小时一个采样点就足够了。但有些酶反应非常快，这需要我们的“添加和读取”动态板成像仪。
在反应的初始速度阶段测量酶活性非常重要。然而，过去对检测信号的持续监测并不总是可行的。FDSS 为 HTS 环境提供了解决方案，尤其是快速反应。
这里的示例均由当时的高端系统 FDSS7000EX 或 FDSS6000 完成。FDSS-GX 继承了所有光学设计，并与 FDSS7000EX 和 FDSS6000 完全兼容。这些测定法正在利用 FDSS-GX 中提供的紫外线激发。
相关科学材料
- 出版物
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作者 标题 来源 Mori T、Itami S、Yanagi T、Tatara Y、Takamiya M、Uchida T。 使用实时荧光监测系统对脯氨酰异构酶抑制剂进行高通量筛选 J Biomol Screen。2009 年 4 月；14(4)：419-24。 Erica VanderPorten、Lauren Frick、Rebecca Turincio、Peter Thana、William LaMarr、Yichin Liu 用于筛选和表征新型乳酸脱氢酶抑制剂的无标记高通量测定 2013 年 10 月 15 日，第 2 期，第 441 卷，第 115–122 页 - 应用说明
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应用 说明第 标题 FDSS 应用说明第 19 FDSS 用途说明 19，快速动力学酶测定脯氨酰异构酶 (Pin1) 抑制剂的高通量筛选 [0.2MB/PDF]
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