L16462-01 是一款集成了灯和集束光纤的光源,用于在近红外分光光度测定中进行漫反射测量。来自光源的光线被照射到样品上,并被漫射和反射。来自光源的光线被照射到样品上,并被漫射和反射。通过连接近红外光谱仪,可以使用导入光纤的光进行光谱分析。通过将多个灯和光纤相互紧密排列,该光源可以高效检测样品发出的微弱漫射光。
使用漫反射光源 L16462-01 和滨松的近红外光谱仪 FTIR 引擎,通过 PLS 回归分析*2 对茶碱浓度不同的 6 种片剂中的茶碱进行定量测定。
通过从先前采集的训练数据*3 生成校准曲线并进行 PLS 回归分析来预测茶碱含量。
校准曲线上的测定系数为 R2=0.998,表明高精度测量是可能的。
*3:当对物质进行定量时,这是通过预先测量标准物质的吸光度来表示相对于浓度变化的数据。
在 1100 nm 至 2000 nm 和 2000 nm 至 2500 nm 这两个波长范围内,使用商业光源和滨松的漫反射光源 L16462-01,通过 PLS 回归分析测量各茶碱浓度,并按上述方法预测精度。
典型的商业光源在 2000 nm 及以上的长波长范围内的定量精度较低。相比之下,滨松的漫反射光源在高达 2500 nm 的近红外区域具有高光子探测效率,因此与商业光源相比,即使在测量 2000 nm 或以上的波长时也能实现高定量精度。
PLS 回归分析 | 商业光源 | 新品 漫反射光源 | ||
---|---|---|---|---|
1100 nm 至 2000 nm | 2000 nm 至 2500 nm | 1100 nm 至 2000 nm | 2000 nm 至 2500 nm | |
测定常数 (R2) | 0.993 | 0.968 | 0.998 | 0.998 |
均方根偏差 (RMSE) | 0.0115 | 0.0144 | 0.0064 | 0.006 |
平均绝对百分比误差 (MAPE) | 5.60% | 6.60% | 2.10% | 1.50% |
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