光电倍增管模块
H15460-40 光电倍增管模块采用 GaAsP 光阴极面光电倍增管。
受光面为 14 mm 正方形,是多光子激发显微镜的理想选择。H15460-40 还包括一个频带为 30 MHz 且电流至电压转换系数为 0.02 V/μA 的放大器。
多光子显微成像技术是一种用于观测紫外线至可见光区域荧光的技术,通过同时吸收两个光子激发荧光分子时,便会出现这种荧光。
共同荧光测定中激发光的波长短于荧光波长。然而,多光子光谱仪在近红外区域使用波长长于荧光波长的激发光。由于近红外光比可见光更容易通过物体,因此它可以提供关于物体内深层部分的信息,并且还可以减少物体内部的散射和背景噪声的影响。此外,近红外光的能量低于可见光和紫外光,从而最大限度地减少对细胞的损害。
该视频显示了在距小鼠脑表面约 500 μm 的深度,第五皮层中超过 6000 个单一神经元的活动。该视频是使用一款装备了 H15460-40 光电传感器模块和专用光学系统的显微镜拍摄的。H15460-40 具有大受光面和高灵敏度,提供了宽视野和高分辨率的观测结果。
到目前为止,用双光子显微镜观测到的视野仅限于约 0.25 mm2 的极小面积。这种非常狭窄的视野阻止了同时观察多个脑区域的神经细胞,即使在观察小鼠等小动物时也是如此。
为了在更宽的视野下进行这项测量,具有大感光面的 H15460-40 光电传感器模块结合使用了设计独特的光学系统。这种设置允许在比传统双光子显微镜大 36 倍的视野中观察细胞活动。
以高时间分辨率观察细胞活动通常需要执行高速激光扫描。然而,提高激光扫描速度会缩短激光在样品上的停留时间,使得难以捕捉具有高信噪比的图像。荧光强度也显著下降,尤其是在样品内执行深层成像时。
为了应对这一问题并捕捉更多的荧光,H15460-40 光电传感器模块具有高灵敏度和大受光面,可与具有独特设计的光学系统结合使用。这允许捕捉更多直到现在才能检测到的荧光,因此即使在样品内进行深层观察时也可以捕捉具有高信噪比的图像。
光阴极面区域形状 | 正方形 |
---|---|
光阴极面区域尺寸 | 14 mm x 14 mm |
波长(短) | 300 nm |
波长(长) | 740 nm |
波长(峰值) | 520 nm |
尺寸 (W x H x D) | 38 mm x 38 mm x 55 mm |
输入电压 | +/-4.5 V 至 +/-5.5 V |
最大值 输入电压 | +/-5.5 V |
最大值 输入电流 | +26 mA / -23 mA |
最大值 输出信号电压 | 2 V |
最大值 控制电压 | 1.1 V |
建议的控制电压调节范围 | +0.5 V 至 +1.0 V |
[阴极] 辐射灵敏度典型值 | 189 mA/W |
[阴极] 亮度灵敏度典型值 | 850 μA/lm |
[阳极] 辐射灵敏度典型值 | 7.5 V/nW |
[阳极] 亮度灵敏度典型值 | 3.4 x 107 V/lm |
[阳极] 输出电压取决于 PMT 暗电流典型值 | 0.2 mV |
[阳极] 输出电压取决于 PMT 暗电流最大值 | 1 mV |
电流至电压转换系数 | 0.02 V/uA |
输出偏移电压典型值 | +/-5 mV |
波纹噪声(峰间)最大值 | 0.5 mV |
建立时间最大值 | 10 s |
工作环境温度 | +5°C 至 +50°C |
储存温度 | -20°C 至 +50°C |
重量 | 98 g |
It looks like you're in the . If this is not your location, please select the correct region or country below.
You're headed to Hamamatsu Photonics website for (Chinese). If you want to view an other country's site, the optimized information will be provided by selecting options below.
In order to use this website comfortably, we use cookies. For cookie details please see our cookie policy.
This website or its third-party tools use cookies, which are necessary to its functioning and required to achieve the purposes illustrated in this cookie policy. By closing the cookie warning banner, scrolling the page, clicking a link or continuing to browse otherwise, you agree to the use of cookies.
Hamamatsu uses cookies in order to enhance your experience on our website and ensure that our website functions.
You can visit this page at any time to learn more about cookies, get the most up to date information on how we use cookies and manage your cookie settings. We will not use cookies for any purpose other than the ones stated, but please note that we reserve the right to update our cookies.
For modern websites to work according to visitor’s expectations, they need to collect certain basic information about visitors. To do this, a site will create small text files which are placed on visitor’s devices (computer or mobile) - these files are known as cookies when you access a website. Cookies are used in order to make websites function and work efficiently. Cookies are uniquely assigned to each visitor and can only be read by a web server in the domain that issued the cookie to the visitor. Cookies cannot be used to run programs or deliver viruses to a visitor’s device.
Cookies do various jobs which make the visitor’s experience of the internet much smoother and more interactive. For instance, cookies are used to remember the visitor’s preferences on sites they visit often, to remember language preference and to help navigate between pages more efficiently. Much, though not all, of the data collected is anonymous, though some of it is designed to detect browsing patterns and approximate geographical location to improve the visitor experience.
Certain type of cookies may require the data subject’s consent before storing them on the computer.
This website uses two types of cookies:
This website uses cookies for following purposes:
Cookies help us help you. Through the use of cookies, we learn what is important to our visitors and we develop and enhance website content and functionality to support your experience. Much of our website can be accessed if cookies are disabled, however certain website functions may not work. And, we believe your current and future visits will be enhanced if cookies are enabled.
There are two ways to manage cookie preferences.
If you don’t want to receive cookies, you can modify your browser so that it notifies you when cookies are sent to it or you can refuse cookies altogether. You can also delete cookies that have already been set.
If you wish to restrict or block web browser cookies which are set on your device then you can do this through your browser settings; the Help function within your browser should tell you how. Alternatively, you may wish to visit www.aboutcookies.org, which contains comprehensive information on how to do this on a wide variety of desktop browsers.
Occasionally, we may use internet tags (also known as action tags, single-pixel GIFs, clear GIFs, invisible GIFs and 1-by-1 GIFs) at this site and may deploy these tags/cookies through a third-party advertising partner or a web analytical service partner which may be located and store the respective information (including your IP-address) in a foreign country. These tags/cookies are placed on both online advertisements that bring users to this site and on different pages of this site. We use this technology to measure the visitors' responses to our sites and the effectiveness of our advertising campaigns (including how many times a page is opened and which information is consulted) as well as to evaluate your use of this website. The third-party partner or the web analytical service partner may be able to collect data about visitors to our and other sites because of these internet tags/cookies, may compose reports regarding the website’s activity for us and may provide further services which are related to the use of the website and the internet. They may provide such information to other parties if there is a legal requirement that they do so, or if they hire the other parties to process information on their behalf.
If you would like more information about web tags and cookies associated with on-line advertising or to opt-out of third-party collection of this information, please visit the Network Advertising Initiative website http://www.networkadvertising.org.
We use third-party cookies (such as Google Analytics) to track visitors on our website, to get reports about how visitors use the website and to inform, optimize and serve ads based on someone's past visits to our website.
You may opt-out of Google Analytics cookies by the websites provided by Google:
https://tools.google.com/dlpage/gaoptout?hl=en
As provided in this Privacy Policy (Article 5), you can learn more about opt-out cookies by the website provided by Network Advertising Initiative:
http://www.networkadvertising.org
We inform you that in such case you will not be able to wholly use all functions of our website.
Close