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CYTOQUBE® 光片微孔板细胞仪
C15200-01RGBU
CYTOQUBE 是一款光片微孔板细胞仪，可对 2D 和 3D 细胞培养样品进行高速荧光成像和分析。它能够扫描 1536、384 和 96 孔微孔板，采集整个孔的 3D 荧光图像。
在图像采集过程中，分析对象（细胞、球状体或类器官）的荧光强度和 3D 形态信息，并在扫描完成后显示结果。
CYTOQUBE 和 Zyncscan是日本滨松光子学株式会社，及其全球子公司（中国大陆、欧洲联盟、日本、瑞士、英国、美国）注册使用的商标。
三个关键特点
CYTOQUBE 具有以下三个特点
- 高通量
- 扫描和分析设置简单
- 3D 荧光成像逼真度和精度增强
这些特点有助于促进转化研究，并提高药物筛选、毒理学和安全性评估的效率。
扫描速度快
无论孔板形式（1536、384、96 孔）或样品深度如何，均可在几分钟内采集每种颜色的整个孔和整个板的荧光图像。
扫描和分析并行处理
荧光图像构建和图像分析的并行处理显著缩短了从开始扫描到输出数据分析结果的时间。
设置简单
这些设置已经简化，易于使用。不包括不必要的设置，例如“焦点调整”或“曝光时间调整”。可以通过导航扫描功能自动设置最佳灵敏度。此外，您可以将从扫描到结果的整个处理显示为协议文件，从而轻松创造扫描和分析条件。
直观的用户界面
可以直观地设置图像分析设置，因为其针对每个用途均汇总了必要的设置。
完整的扫描和分析软件集成在一个封装中
单个软件可用于检查每个孔的 2D/3D 荧光图像、计算 EC50/IC50、创建各种图表、识别命中孔并生成简单报告。
3D 荧光图像显示
散点图和门控设置
命中孔识别
实时背景分离
实时分离源自溶液中培养液或荧光染料自发荧光的背景荧光和源自样品的荧光与所采集的 XZ 断层荧光图像，只能有效去除三维背景荧光。
在荧光显微镜中使用 CYTOQUBE 测量以含有血清和荧光染料的 DMEM 培养液培养的 A549 细胞，来比较图像。
荧光染料：Annexin-V-Alexa Fluor 488
关闭实时背景分离
开启实时背景分离
无缝 3D 荧光图像
在典型的共聚焦显微镜中，采集不同深度的多个 2D XY 图像来构建 3D 荧光图像。要获得更准确的 3D 信息，需要采集更多图像。
CYTOQUBE 始终使用 Zyncscan 技术提供无缝 3D 荧光图像，无需进行大量图像采集即可获得精确的 3D 信息。
微型应用说明
详细参数
| 产品名称 | CYTOQUBE 光片微孔板细胞仪 |
|---|---|
| 类型编号 | C15200-01RGBU |
| 扫描方法 | 使用光片照明的高速 3D 荧光测定方法 (ZyncscanⓇ) |
| 激发激光器 | 从 405 nm、488 nm、561 nm 和 637 nm 中选择（最多可选择 4 种类型） |
| 荧光滤波器 | 从 458 nm、531 nm、600 nm 和 680 nm 中选择（最多可安装 6 套） |
| 成像设备 | 科学 CMOS 图像传感器 |
| 图像分辨率 | 2.75 μm (X) × 2.75 μm (Y) × 6.215 μm (Z) |
| 最大可观察高度*1 | 400 μm |
| 焦点 | 免聚焦 (Zyncscan) |
| 微孔板 | 1536、384、96 孔微孔板（SBS 标准规格） |
| 温度设置范围 | +30 °C 至 +45 °C（环境温度：+25 °C） |
| CO2浓度监测范围 | 0 % 至 10 %（带 CO2 入口） |
| 条码扫描器 | 1D 条形码（读取位置：微孔板短边的两侧）*2 |
| 电源 | AC 100 V 至交流 240 V |
| 用电功耗 | Approx. 700 VA |
| 操作环境温度 | +20 ̊C 至 +30 ̊C |
| 操作环境湿度 | 30% 至 80%（无雾气现象） |
*1 微孔板底于短边方向上的平整度为 ± 50 μm 时的距孔底高度
*2 厚度小于 9.5 mm 的 1536、384 和 96 板可能无法正确读取。
* 如需自动化功能信息，请联系我们的销售代表。
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