FDSS®-GX 动态板成像仪
FDSS-GX 是 FDSS 系列中性能最高的动态板成像仪,针对 96 孔、384 孔和 1536 孔进行了优化,可实现可靠而又稳定的高通量筛选 (HTS),从而实现整个微孔板成像和同时向所有孔进样。该系统使用 qCMOS 传感器实现高灵敏度成像,并通过采用能够分配可变体积的 1536 通道分配装置,实现高精度和可重复的微量分配。通过组合可选部件,可以根据用户的目的构建无机器人半自动化系统。
FDSS是日本滨松光子学株式会社,及其全球子公司(中国大陆、法国、德国、意大利、日本、英国、美国)注册使用的商标。
分配准确性和可重复性是进行分析的重要因素。
FDSS-GX 通过采用能够分配可变体积的 1536 通道分配装置,实现了高精度和可重复的微量分配。该分配装置具有专用于 FDSS-GX 的独立金属活塞缸和分配头。
还提供 96 通道和 384 通道的分配装置。
分配装置(1536 孔类型)A15623-28
用于 FDSS-GX A8687-82 的 1536 黑色吸头(10 个支架)
下图显示了将 B 珠分配到 1536 微孔板(5 µL/孔)中时荧光强度随时间和板图像的变化,显示分配后的 CV 为 1.89%。
1536 通道分配装置带有独立的金属活塞缸和专用于 FDSS 的分配头,提供最佳的分配性能。
*这是使用经过检修或校准的 1536 通道分配装置和专用于 FDSS-GX 的分配头分配 B 珠(5 µL/孔)时的实际测量值。
高灵敏度 qCMOS 传感器是 FDSS-GX 光学系统的标准配置。
在荧光和发光测量中,低光成像实现了高定量性能,并且由于传感器的宽动态范围,检测窗口已经扩大,从而实现了宽线性范围的测量。
此外,由于传感器尺寸大,qCMOS 传感器可以对整个板进行成像,并实现高分辨率测量。
在荧光测定中,FDSS-GX 能够检测 0.03 nM 荧光素*(曝光时间:50 ms)。
下图显示了荧光素滴定浓度的荧光计数和信噪比,图像显示了 384 微孔板。
* 这是实际测量值。
荧光素浓度与 RFU
信噪比
微孔板图像
(384 微孔板)
在发光测量中,根据计算,FDSS-GX 能够检测到 3.9 fg 荧光素酶*(曝光时间:5 秒)。
下图显示了荧光素酶滴定浓度的发光计数,图像显示了 384 微孔板。
* 这是根据以下测量数据计算的理论值。
荧光素酶(pg/孔)与 RLU
微孔板图像
(384 微孔板)
由于传感器尺寸大,qCMOS 传感器可以对整个板进行成像,并实现高分辨率测量。
(1536 板,样品:B 珠)
FDSS-GX 包括高精度荧光光学系统,采用内部开发的大功率、长寿命氙光源。氙光源与专用二向色镜/光学滤光片相结合,可使用 UV 至 IR 范围内的各种荧光染料进行测量。
有关详细信息,请参阅下表。
落射荧光光学系统
*:选项
上表中的波长值可能不对应于每个用途的典型激发/发光波长。
根据用途,我们提供适用于 FDSS-GX 光学系统的滤波器组。请联系您的滨松代表了解更多信息。
FDSS-GX 可以通过前后两条传输线自动传输化验板和化合物板。此外,带有大容量盒的堆板装置可容纳多达 20 个微孔板,可用于无机器人半自动化。
FDSS-GX 内部布局
堆板装置
使用 Fluo-8 AM 染色的 CHO 细胞评估 ATP 剂量反应
*可使用 Tl+ 进行钾离子通道筛选。
使用 FluxOR 在 CHO 细胞中进行 K+ 测定
使用 FRET 型电压敏感染料 (VSP) 评估 Nav 1.5-CHO 细胞
使用 iPS 细胞衍生的心肌细胞进行 Ca2+ 瞬时和膜电位测量
使用表达水母发光蛋白的细胞系通过发光进行细胞内 Ca2+ 测定
使用表达水母蛋白的细胞进行 Ca2+ 测定
使用 CHO 细胞进行 BRET 测定
β-抑制蛋白 BRET1- 测定原理
Rluc - D2s 受体和 eYFP - β-抑制蛋白 2 在 CHO 细胞中表达,并检测到加入配体后细胞中出现了 Rluc 和 EYFP BRET。
使用 GloSensor 进行 cAMP 评估
使用表达 GloSensor (Promega) 的 HUVEC 分析 cAMP 的时程
使用 CytoTox-Glo 细胞毒性测定法评估细胞数量
* 使用 FDSS 软件选项时 高速采集选件 U8524-11
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