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FDSS®-GX 动态板成像仪
C15711-02
FDSS-GX 是 FDSS 系列中性能最高的动态板成像仪，针对 96 孔、384 孔和 1536 孔进行了优化，可实现可靠而又稳定的高通量筛选 (HTS)，从而实现整个微孔板成像和同时向所有孔进样。该系统使用 qCMOS 传感器实现高灵敏度成像，并通过采用能够分配可变体积的 1536 通道分配装置，实现高精度和可重复的微量分配。通过组合可选部件，可以根据用户的目的构建无机器人半自动化系统。
FDSS是日本滨松光子学株式会社，及其全球子公司（中国大陆、法国、德国、意大利、日本、英国、美国）注册使用的商标。
特点
1. 1536 通道歧管移液器头，带独立金属活塞缸
分配准确性和可重复性是进行分析的重要因素。
FDSS-GX 通过采用能够分配可变体积的 1536 通道分配装置，实现了高精度和可重复的微量分配。该分配装置具有专用于 FDSS-GX 的独立金属活塞缸和分配头。
还提供 96 通道和 384 通道的分配装置。
分配装置（1536 孔类型）A15623-28
用于 FDSS-GX A8687-82 的 1536 黑色吸头（10 个支架）
● 分配性能
下图显示了将 B 珠分配到 1536 微孔板（5 µL/孔）中时荧光强度随时间和板图像的变化，显示分配后的 CV 为 1.89%。
1536 通道分配装置带有独立的金属活塞缸和专用于 FDSS 的分配头，提供最佳的分配性能。
*这是使用经过检修或校准的 1536 通道分配装置和专用于 FDSS-GX 的分配头分配 B 珠（5 µL/孔）时的实际测量值。
2. 使用 qCMOS® 传感器进行高灵敏度和高分辨率成像
高灵敏度 qCMOS 传感器是 FDSS-GX 光学系统的标准配置。
在荧光和发光测量中，低光成像实现了高定量性能，并且由于传感器的宽动态范围，检测窗口已经扩大，从而实现了宽线性范围的测量。
此外，由于传感器尺寸大，qCMOS 传感器可以对整个板进行成像，并实现高分辨率测量。
● 荧光探测效率
在荧光测定中，FDSS-GX 能够检测 0.03 nM 荧光素*（曝光时间：50 ms）。
下图显示了荧光素滴定浓度的荧光计数和信噪比，图像显示了 384 微孔板。
* 这是实际测量值。
荧光素浓度与 RFU
信噪比
微孔板图像
（384 微孔板）
● 发光探测效率
在发光测量中，根据计算，FDSS-GX 能够检测到 3.9 fg 荧光素酶*（曝光时间：5 秒）。
下图显示了荧光素酶滴定浓度的发光计数，图像显示了 384 微孔板。
* 这是根据以下测量数据计算的理论值。
荧光素酶（pg/孔）与 RLU
微孔板图像
（384 微孔板）
● 整板成像比较
由于传感器尺寸大，qCMOS 传感器可以对整个板进行成像，并实现高分辨率测量。
（1536 板，样品：B 珠）
3. 具有大功率和长寿命氙光源的高精度落射荧光光学系统
FDSS-GX 包括高精度荧光光学系统，采用内部开发的大功率、长寿命氙光源。氙光源与专用二向色镜/光学滤光片相结合，可使用 UV 至 IR 范围内的各种荧光染料进行测量。
有关详细信息，请参阅下表。
落射荧光光学系统
| 用户用途 | ||||||
|---|---|---|---|---|---|---|
| 激发滤波器 1 | 激发滤波器 2 | 短通滤波器 | 二向色镜 | 发光滤波器 1 | 发光滤波器 2 | |
| Fura-2 (Ca2+) | 340 nm* | 387 nm* | SPF 495 | UV* | 520 nm ～ 560 nm | - |
| SBFI (Na+) | 340 nm* | 387 nm* | SPF 495 | UV* | 520 nm ～ 560 nm | - |
| PBFI (K+) | 340 nm* | 387 nm* | SPF 495 | UV* | 520 nm ～ 560 nm | - |
| MQAE，diH-MEQ (CI-) | 387 nm* | - | SPF 495 | UV* | 440 nm ～ 470 nm* | - |
| DAPI (DNA) | 387 nm* | - | SPF 495 | UV* | 440 nm ～ 470 nm* | - |
| Hoechst33258 | 387 nm* | - | SPF 495 | UV* | 440 nm ～ 470 nm* | - |
| VSP-1 | 387 nm* | - | SPF 400* | 用于 VSP* | 466 nm* | 560 nm* |
| CFP/YFP | 438 nm* | - | SPF 450* | 用于 C/Y* | 483 nm* | 542 nm* |
| HYPER | 425 nm* | 483 nm* | SPF 495 | 用于 HYPER* | 520 nm ～ 560 nm | - |
| Fluo-8 (Ca2+) | 472 nm | - | SPF 495 | B | 520 nm ～ 560 nm | - |
| Cal-520 (Ca2+) | 472 nm | - | SPF 495 | B | 520 nm ～ 560 nm | - |
| Sodium Green (Na+) | 472 nm | - | SPF 495 | B | 520 nm ～ 560 nm | - |
| FluxOR (K+) | 472 nm | - | SPF 495 | B | 520 nm ～ 560 nm | - |
| BCECF (pH) | 472 nm | - | SPF 495 | B | 520 nm ～ 560 nm | - |
| FluoVolt | 472 nm | - | SPF 495 | B | 520 nm ～ 560 nm | - |
| GFP | 472 nm | - | SPF 495 | B | 520 nm ～ 560 nm | - |
| FITC | 472 nm | - | SPF 495 | B | 520 nm ～ 560 nm | - |
| YFP | 472 nm | - | SPF 495 | 用于 YFP* | 520 nm ～ 560 nm | - |
| JC-1 | 531 nm | - | SPF 550 | 用于 JC-1* | 593 nm | - |
| CoroNa Red (Na+) | 531 nm | - | SPF 550 | 用于 FMP | 593 nm | - |
| FMP | 531 nm | - | SPF 550 | 用于 FMP | 593 nm | - |
| Cal-590 (Ca2+) | 531 nm | - | SPF 550 | 用于 FMP | 593 nm | - |
| Rhodamine | 531 nm | - | SPF 550 | 用于 FMP | 593 nm | - |
| Cal-630 (Ca2+) | 605 nm* | - | SPF 630* | 用于 RED* | 676 nm* | - |
| Nano-BRET | - | - | - | - | 461 nm* | 647 nm* |
*：选项
上表中的波长值可能不对应于每个用途的典型激发/发光波长。
根据用途，我们提供适用于 FDSS-GX 光学系统的滤波器组。请联系您的滨松代表了解更多信息。
4. 用于半自动化的堆板装置
FDSS-GX 可以通过前后两条传输线自动传输化验板和化合物板。此外，带有大容量盒的堆板装置可容纳多达 20 个微孔板，可用于无机器人半自动化。
FDSS-GX 内部布局
堆板装置
用途
荧光用途
● Ca2+ 测定
Fluo-8、Calbryte、-520、-590、-630 AM、Fura-2
使用 Fluo-8 AM 染色的 CHO 细胞评估 ATP 剂量反应
● 膜电位测定
FluoVolt、SQMP、FMP
● 其他离子通道测定
K+：FluxOR*、Na+：ANG2、Sodium Green、Cl-：YFP、diH-MEQ
*可使用 Tl+ 进行钾离子通道筛选。
使用 FluxOR 在 CHO 细胞中进行 K+ 测定
● FRET 测定
CFP/YFP、电压传感器探针、荧光探针、荧光蛋白
使用 FRET 型电压敏感染料 (VSP) 评估 Nav 1.5-CHO 细胞
● iPSC 衍生细胞
使用 iPS 细胞衍生的心肌细胞进行 Ca2+ 瞬时和膜电位测量
发光用途
● Ca2+ 测定
发光蛋白
使用表达水母发光蛋白的细胞系通过发光进行细胞内 Ca2+ 测定
使用表达水母蛋白的细胞进行 Ca2+ 测定
● BRET 测定
BRET1、BRET2、NanoBRET
使用 CHO 细胞进行 BRET 测定
β-抑制蛋白 BRET1- 测定原理
Rluc - D2s 受体和 eYFP - β-抑制蛋白 2 在 CHO 细胞中表达，并检测到加入配体后细胞中出现了 Rluc 和 EYFP BRET。
● cAMP 测定
GloSensor
使用 GloSensor 进行 cAMP 评估
使用表达 GloSensor (Promega) 的 HUVEC 分析 cAMP 的时程
● 使用 CytoTox-Glo 细胞毒性测定法评估细胞数量
使用 CytoTox-Glo 细胞毒性测定法评估细胞数量
详细参数
| 分配装置 | 1536 孔类型 A15623-28 | 1 μL 至 5 μL |
|---|---|---|
| 384 孔类型 A10118-26 | 1 μL 至 30 μL | |
| 96 孔类型 A10118-24 | 10 μL 至 200 μL | |
| 荧光和发光探测器 | 专用于 FDSS 的滨松 qCMOS 传感器 | |
| 采样数据点数量 | 1 至 50000 次采样 | |
| 采样率 | 最低 10 Hz（每秒 10 个数据点） 最高 120 Hz（每秒 120 个数据点）* |
|
| 采样间隔 | 最少 0.1 秒 最少 0.0083 秒* |
|
| 激发光源 | 氙气灯 可以选择滤光片 |
|
| 板抖动功能 | 256 步长可调 每分钟转数：0~3000 rpm |
|
| 加热器装置 | 温度控制部件 | FDSS-GX 主装置室（整个上半部分） |
| 可配置温度 | 最高 +40 ºC | |
| 吸头装载 | 使用自动吸头装载器 A15623-07 | |
| 板装载线数 | 前装载机 × 1 后装载机 × 1 | |
| 板位数 | 测定板 | 前装载机 × 1 |
| 复合板 | 后装载机×1，复合板级（手动交换）× 3 | |
| 吸头清洗器位置数 | 最多 3 个装置（具有/不具有大功率超声功能的总和，深容器） | |
| 分配装置位置数 | 最多 2 个装置（仅限相同吸头类型） | |
| 兼容的分配头 | 专用于 FDSS、A8687 系列的 1536/384/96 黑色吸头 一次性类型 |
|
| 兼容微孔板 | 透明底部黑色 1536/384/96 板 SBS 格式高度至少 10 mm |
|
| 电源规格 | 单相交流 100 V ~ 240 V，50 Hz/60 Hz | |
| 功耗 | C15711-02：约 3115 VA | |
| C7903-13：约 660 W | ||
| 所需供电线路：15 A × 3 线 | ||
| 环境工作温度 | +15°C 至 30°C | |
| 尺寸/重量 | 主机 | 1437 (W) × 850 (D) × 1467 (H) mm/约 320 kg |
| 数据分析装置 | 570 (W) × 880 (D) × 943 (H) mm/约 82 kg | |
| 堆板装置 | 437 (W) × 580 (D) × 1340 (H) mm/约 36 kg | |
| 清洗架 | 616 (W) × 924 (D) × 1038 (H) mm/约 19 kg | |
| 循环水冷却器 | 222 (W) × 386 (D) × 649 (H) mm/约 26 kg | |
| 自动吸头装载器 | 233 (W) × 289 (D) × 447 (H) mm/约 21 kg | |
* 使用 FDSS 软件选项时 高速采集选件 U8524-11
外形尺寸图
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