CYTOQUBEⓇ 具有以下三个特点
这些特点有助于提高使用细胞、球状体、类器官和生物打印样品进行药物筛选、毒性/安全性评估和转化研究的效率。
无论孔板形式(1536、384、96 孔)或样品深度如何,均可在几分钟内采集每种颜色的整个孔和整个板的荧光图像。
荧光图像构建和图像分析的并行处理显著缩短了从开始扫描到输出数据分析结果的时间。
这些设置已经简化,易于使用。不包括不必要的设置,例如“焦点调整”或“曝光时间调整”。可以通过导航扫描功能自动设置最佳灵敏度。此外,您可以将从扫描到结果的整个处理显示为协议文件,从而轻松创造扫描和分析条件。
可以直观地设置图像分析设置,因为其针对每个用途均汇总了必要的设置。
单个软件可用于检查每个孔的 2D/3D 荧光图像、计算 EC50/IC50、创建各种图表、识别命中孔并生成简单报告。
3D 荧光图像显示
散点图和门控设置
命中孔识别
实时分离源自溶液中培养液或荧光染料自发荧光的背景荧光和源自样品的荧光与所采集的 XZ 断层荧光图像,只能有效去除三维背景荧光。
在荧光显微镜中使用 CYTOQUBE 测量以含有血清和荧光染料的 DMEM 培养液培养的 A549 细胞,来比较图像。
荧光染料:Annexin-V-Alexa Fluor 488
关闭实时背景分离
开启实时背景分离
在典型的共聚焦显微镜中,采集不同深度的多个 2D XY 图像来构建 3D 荧光图像。要获得更准确的 3D 信息,需要采集更多图像。
CYTOQUBE 始终使用 ZyncscanⓇ 技术提供无缝 3D 荧光图像,无需进行大量图像采集即可获得精确的 3D 信息。
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