MAICO具有独特的子装置结构,在单一的装置中包含了各个荧光波段激发和检测所需的一切组件。
通过采用 子装置结构,您可以在购买第一个装置时选择(比如)单个荧光带。 然后,随着研究的进展,您可以添加更多的成像通道。
MAICO支持从单通道观察,到多达四个的多通道(405 nm、488 nm、561 nm、638 nm)同步激发和观察。
滨松光子学株式会社的探测器在荧光测定领域拥有长久的业务记录。通过采用世界上最灵敏的探测器(光电倍增管)并应用我们的信号处理技术,我们能够将激光功率降低到具有足够荧光信号的 3R 级。
这对于活细胞成像具有重要影响,例如能够实现长延时成像。
我们采用 29 kHz 谐振型高速 MEMS 振镜作为点扫描设备。MEMS 振镜可实现高达 76 帧/秒的高速扫描,并可用于钙离子动力学等高速现象。
高速谐振扫描系统减少了激光辐照时间,为活细胞和固定样品降低光毒性,减轻光漂白,并实现高效观察。
高速扫描可实现高分辨率的舒适观察,在搜索和聚焦样品时,显示延迟微乎其微。
MAICO的设计经过了很长的发展历程,符合传统的共聚焦光学器件标准,使用点扫描设备和针孔来获取光学切片图像。
在为较厚的荧光样品成像时,只有从物镜的焦点平面发射的荧光通过针孔到达探测器,而从远离焦点平面的地方发射的荧光则会被阻挡。
它检测可定量、可再现且可靠的信号。它获取的还是高对比度图像,因而不需要去卷积等图像处理技术。
由于 MAICO利用了我们最灵敏的探测器和信号处理技术,我们能够成功地将激光功率降低到 3R 级。因此,您可以在常规实验室环境中使用 MAICO,而不需要激光控制区域。
MAICO 兼容 DCAM-API,这是一种常见的相机库,可通过滨松的 HCImage 软件加以控制。也可以使用兼容 DCAM-API的第三方软件采集图像。
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