超分辨率显微镜是指获得空间分辨率高于衍射极限的显微镜图像的一系列方法。超分辨率显微镜需要配备兼具极低噪声和小像素尺寸,从而产生更高分辨率的科学相机。
ORCA-Quest 生成的超高分辨率图像
qCMOS 相机 / 4.6 μm 像素尺寸
ORCA-Fusion 生成的超高分辨率图像
Gen III sCMOS 相机/6.5 μm 像素尺寸
使用 ORCA-Quest 进行实验设置
由 Visitech International 的 Steven Coleman 提供,该图像采用 VT-iSIM 这种高速超分辨率活细胞成像系统生成。
与传统荧光显微镜相比,生物发光显微镜具有独特优势(例如不需要激发光),因而备受关注。生物发光的主要缺点是其非常低的光强度,这会导致曝光时间长、图像质量低。生物发光研究即使在长时间曝光时也需要高灵敏度相机。
NanoLuc 融合蛋白质 ARRB2 和 Venus 融合蛋白质 V2R 位于附近,即将发生 BRET。
视场整体图像(物镜:20× / 曝光时间:30 秒 / 像素合并:4×4)
显微镜系统的外观
数据提供:东北大学分子和细胞生物化学研究生院柳川正隆博士
植物会释放极小一部分光能量,在一段时间内作为光进行光合作用。这种现象被称为延迟荧光。通过检测这种微弱光,可以观察化学物质、病原体、环境和其他应激源对植物的影响。
观赏植物的延迟荧光(在激发光淬灭 10 秒后曝光 10 秒)
当从地面观察星星时,由于大气湍流,星星的图像可能模糊,因此大大降低捕获清晰图像的能力。但是,由于曝光时间短且大气条件适宜,有时可以拍摄到清晰的图像。因此,幸运成像是一种获取大量图像并在对齐时仅整合最清晰的图像的方法。
Orion Nebula(带 3 个波长滤光片的彩色图像)
成像设置
通过自适应光学方法,系统可立即校正受大气波动干扰的入射光的波前。为了执行实时和高精度的波前校正,相机必需以高速和高空间分辨率获取图像。此外,由于波前校正是在测量激光导星的极暗状态下执行的,因此相机还需要具备高灵敏度。
通过自适应光学进行波前校正
自适应光学比较*
*数据提供:京都大学天文系山本广大博士
对于 X 射线或其他类型的高能粒子的成像,通常使用连接了闪烁体的科学相机。成像系统需要低噪声和高速度来检测瞬时现象。
小鼠胚胎 X 射线相差 CT 图像
ORCA-Quest 结合高分辨率 X 射线成像系统 (M11427) 生成的小鼠胚胎的 X 射线相差 CT 图像
曝光时间:15 毫秒,总测量时间:6.5 分钟
实验设置
相机设置
数据提供:日本同步加速器辐射研究所 (JASRI) 高级研究员 Masato Hoshino 博士对 SPring-8 BL20B2 光束线进行的研究
拉曼效应是波长不同于入射光的光散射,拉曼光谱仪是一种通过测量该波长来确定材料特性的技术。拉曼光谱仪可在分子层面进行结构分析,提供化学键合、结晶度等信息。
线扫描类型拉曼成像系统中每像素光子数相等条件下的拉曼光谱(单帧)比较
拉曼图像
qCMOS
EM-CCD
@10 光子/像素/帧,532 nm 激光激发
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