超分辨率显微镜是指一系列获取空间分辨率高于衍射界限的显微镜图像的方法。超分辨率显微镜需要具有极低噪声和小像素尺寸的科学相机,从而实现更高的分辨率。
ORCA®-Quest生成的超高分辨率图像
qCMOS相机 / 4.6 μm像素尺寸
ORCA-Fusion生成的超高分辨率图像
Gen III sCMOS相机/6.5 μm像素尺寸
使用ORCA-Quest进行实验设置
数据由 VisiTech International 的 Steven Coleman 提供,该图像采用 VT-iSIM 这种高速超分辨率活细胞成像系统/仪器生成。
生物发光显微镜因其相对于传统荧光显微镜的独特优势(如无需激发光)而备受关注。生物发光的主要缺点是光强度极低,导致曝光时间长且图像质量低。生物发光调查研究需要高灵敏度相机,甚至长时间曝光也是如此。
同时双波长发光成像
NanoLuc 融合蛋白质 ARRB2 和 Venus 融合蛋白质 V2R 位于附近,即将发生 BRET。
相机:ORCA-Quest + W-VIEW GEMINI
目标:20× / 曝光时间:30 秒 / 像素合并:4×4
显微镜系统的外观
数据来源:Dr. Masataka Yanagawa, Department of Molecular & Cellular Biochemistry Graduate School of Pharmaceutical Science, Tohoku University
植物会释放很小一部分光能量,在一段时间内作为光进行光合作用。这种现象被称为延迟荧光。通过检测这种微弱光,可以观察化学物质、病原体、环境和其他应激源对植物的影响。
观赏植物的延迟荧光(在激发光淬灭10秒后曝光10秒)
Tokai National Higher Education and Research System下属Gifu University的Institute for Glyco-core Research (iGCORE) 之中的Cell Biophysics Laboratory, 正在研究解析细胞内及细胞膜表面分子的作用机制。2022年,该实验室引进ORCA-Quest作为单分子荧光观测相机。
我们采访了该实验室的Kenichi Suzuki教授和Koichiro Hirosawa研究员,以及曾任实验室成员(至2023年5月)、现任National Cancer Center Research Institute下属Advanced Bioimaging Research Division主任的Rinshi Kasai,了解他们引进ORCA-Quest的原因、使用体验以及对未来研究的展望。
生物发光显微技术的独特优势促使研究人员挑战传统的荧光方法。在活体细胞与动物的检测和成像中,生物发光报告基因技术被证明是一种具有潜力的方法,在神经科学研究领域尤其如此。
Michael Krieg 博士及其协作团队(来自位于西班牙卡斯特尔德费尔斯的Institut de Ciencies Fotòniques (ICFO) ),揭示了生物发光显微技术在观测秀丽隐杆线虫和其他生物学模式生物方面的优势和极限突破,论证了快速细胞动力学的体积成像。
Drew Robson博士目前在德国Max Planck Institute for Biological Cybernetics从事研究工作,他系统地开发了各种方法,使用定量光子数分辨相机观测斑马鱼幼体在自然行为过程中的大脑功能。
当从地面观察星星时,由于大气湍流,星星的图像可能模糊,因此大大降低捕获清晰图像的能力。但是,由于曝光时间短且大气条件适宜,有时可以拍摄到清晰的图像。因此,幸运成像是一种获取大量图像并在对齐时仅整合最清晰的图像的方法。
Orion Nebula(带3个波长滤光片的彩色图像)
成像设置
通过自适应光学方法,系统可立即校正受大气波动干扰的入射光的波前。为了执行实时和高精度的波前校正,相机必需以高速和高空间分辨率获取图像。此外,由于波前校正是在测量激光导星的极暗状态下执行的,因此相机还需要具备高灵敏度。
通过自适应光学进行波前校正
自适应光学比较
数据来源:Kodai Yamamoto, Ph.D., Department of Astronomy, Kyoto University
The University of Electro-Communications下属Department of Communication Engineering and Informatics的Hosokawa Laboratory使用高灵敏度相机观测极光。极光观测需要高帧率来观测形态和动态,需要高灵敏度来检测暗极光,需要低噪声来顺畅分析获取的图像,并且需要大尺寸传感器来实现宽场成像。ORCA-Quest qCMOS®相机解决了这些问题。
对于 X 射线或其他高能粒子的成像,通常使用连接了闪烁体的科学相机。成像系统必须需要低噪声和高速度来检测瞬时现象。
小鼠胚胎X射线相差CT图像
ORCA-Quest结合高分辨率X射线成像系统 (M11427) 生成的小鼠胚胎的X射线相差CT图像
曝光时间:15毫秒,总测量时间:6.5分钟
实验设置
相机设置
数据来源:SPring-8 BL20B2 beamline by Dr. Masato Hoshino, Senior scientist in Japan Synchrotron Radiation Research Institute (JASRI)
Japan Synchrotron Radiation Research Institute (JASRI)为SPring-8大型同步辐射设施的运行、维护和利用提供技术支持。该机构还支持使用SACLA和NanoTerasu。其中,散射与成像部显微动态成像团队负责开发利用同步辐射的X射线成像系统,并为想要获取X射线显微图像的研究人员提供技术支持。ORCA®-Quest qCMOS®相机被用作该X射线显微成像系统的探测器。
National Institute of Advanced Industrial Science and Technology (AIST)下属National Metrology Institute of Japan (NMIJ) 的Research Institute for Measurement and Analytical Instrumentation之中的Advanced Beam Measurement Group,正在致力于开发用于先进重离子放射治疗的α射线成像技术。这项研究使用ORCA®-Quest qCMOS®相机作为探测器。
拉曼效应是波长不同于入射光的光散射。拉曼光谱仪是一种通过测量该波长来确定材料特性的技术。这种光谱仪可在分子层面进行结构分析,提供化学键合、结晶度等方面的信息。
线扫描类型拉曼成像系统中每像素光子数相等条件下的拉曼光谱(单帧)比较
拉曼图像
qCMOS相机
EM-CCD相机
@10光子/像素/帧,532 nm激光激发
It looks like you're in the . If this is not your location, please select the correct region or country below.
You're headed to Hamamatsu Photonics website for (Chinese). If you want to view an other country's site, the optimized information will be provided by selecting options below.
In order to use this website comfortably, we use cookies. For cookie details please see our cookie policy.
This website or its third-party tools use cookies, which are necessary to its functioning and required to achieve the purposes illustrated in this cookie policy. By closing the cookie warning banner, scrolling the page, clicking a link or continuing to browse otherwise, you agree to the use of cookies.
Hamamatsu uses cookies in order to enhance your experience on our website and ensure that our website functions.
You can visit this page at any time to learn more about cookies, get the most up to date information on how we use cookies and manage your cookie settings. We will not use cookies for any purpose other than the ones stated, but please note that we reserve the right to update our cookies.
For modern websites to work according to visitor’s expectations, they need to collect certain basic information about visitors. To do this, a site will create small text files which are placed on visitor’s devices (computer or mobile) - these files are known as cookies when you access a website. Cookies are used in order to make websites function and work efficiently. Cookies are uniquely assigned to each visitor and can only be read by a web server in the domain that issued the cookie to the visitor. Cookies cannot be used to run programs or deliver viruses to a visitor’s device.
Cookies do various jobs which make the visitor’s experience of the internet much smoother and more interactive. For instance, cookies are used to remember the visitor’s preferences on sites they visit often, to remember language preference and to help navigate between pages more efficiently. Much, though not all, of the data collected is anonymous, though some of it is designed to detect browsing patterns and approximate geographical location to improve the visitor experience.
Certain type of cookies may require the data subject’s consent before storing them on the computer.
This website uses two types of cookies:
This website uses cookies for following purposes:
Cookies help us help you. Through the use of cookies, we learn what is important to our visitors and we develop and enhance website content and functionality to support your experience. Much of our website can be accessed if cookies are disabled, however certain website functions may not work. And, we believe your current and future visits will be enhanced if cookies are enabled.
There are two ways to manage cookie preferences.
If you don’t want to receive cookies, you can modify your browser so that it notifies you when cookies are sent to it or you can refuse cookies altogether. You can also delete cookies that have already been set.
If you wish to restrict or block web browser cookies which are set on your device then you can do this through your browser settings; the Help function within your browser should tell you how. Alternatively, you may wish to visit www.aboutcookies.org, which contains comprehensive information on how to do this on a wide variety of desktop browsers.
Occasionally, we may use internet tags (also known as action tags, single-pixel GIFs, clear GIFs, invisible GIFs and 1-by-1 GIFs) at this site and may deploy these tags/cookies through a third-party advertising partner or a web analytical service partner which may be located and store the respective information (including your IP-address) in a foreign country. These tags/cookies are placed on both online advertisements that bring users to this site and on different pages of this site. We use this technology to measure the visitors' responses to our sites and the effectiveness of our advertising campaigns (including how many times a page is opened and which information is consulted) as well as to evaluate your use of this website. The third-party partner or the web analytical service partner may be able to collect data about visitors to our and other sites because of these internet tags/cookies, may compose reports regarding the website’s activity for us and may provide further services which are related to the use of the website and the internet. They may provide such information to other parties if there is a legal requirement that they do so, or if they hire the other parties to process information on their behalf.
If you would like more information about web tags and cookies associated with on-line advertising or to opt-out of third-party collection of this information, please visit the Network Advertising Initiative website http://www.networkadvertising.org.
We use third-party cookies (such as Google Analytics) to track visitors on our website, to get reports about how visitors use the website and to inform, optimize and serve ads based on someone's past visits to our website.
You may opt-out of Google Analytics cookies by the websites provided by Google:
https://tools.google.com/dlpage/gaoptout?hl=en
As provided in this Privacy Policy (Article 5), you can learn more about opt-out cookies by the website provided by Network Advertising Initiative:
http://www.networkadvertising.org
We inform you that in such case you will not be able to wholly use all functions of our website.
Close