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案例研究 | qCMOS®相机
生命科学
Tokai National Higher Education and Research System下属Gifu University的Institute for Glyco-core Research (iGCORE) 之中的Cell Biophysics Laboratory, 正在研究解析细胞内及细胞膜表面分子的作用机制。2022年，该实验室引进ORCA-Quest作为单分子荧光观测相机。
我们采访了该实验室的Kenichi Suzuki教授和Koichiro Hirosawa研究员，以及曾任实验室成员（至2023年5月）、现任National Cancer Center Research Institute下属Advanced Bioimaging Research Division主任的Rinshi Kasai，了解他们引进ORCA-Quest的原因、使用体验以及对未来研究的展望。
生物发光显微技术的独特优势促使研究人员挑战传统的荧光方法。在活体细胞与动物的检测和成像中，生物发光报告基因技术被证明是一种具有潜力的方法，在神经科学研究领域尤其如此。
Michael Krieg 博士及其协作团队（来自位于西班牙卡斯特尔德费尔斯的Institut de Ciencies Fotòniques (ICFO) ），揭示了生物发光显微技术在观测秀丽隐杆线虫和其他生物学模式生物方面的优势和极限突破，论证了快速细胞动力学的体积成像。
Drew Robson博士目前在德国Max Planck Institute for Biological Cybernetics从事研究工作，他系统地开发了各种方法，使用定量光子数分辨相机观测斑马鱼幼体在自然行为过程中的大脑功能。
为了达到最佳单分子成像性能，Chinese Academy of Sciences下属Institute of Genetics and Developmental Biology的Jiachao Xu博士搭建了TIRF显微镜，并在最近几年不断改进。近期，Xu博士在其活细胞血管紧张素II 1型受体 (AT1R) 单分子荧光成像实验中，比较了EM-CCD与qCMOS 两种相机的性能。
天文学
The University of Electro-Communications下属Department of Communication Engineering and Informatics的Hosokawa Laboratory使用高灵敏度相机观测极光。极光观测需要高帧率来观测形态和动态，需要高灵敏度来检测暗极光，需要低噪声来顺畅分析获取的图像，并且需要大尺寸传感器来实现宽场成像。ORCA-Quest qCMOS®相机解决了这些问题。
高能物理同步辐射
Japan Synchrotron Radiation Research Institute (JASRI)为SPring-8大型同步辐射设施的运行、维护和利用提供技术支持。该机构还支持使用SACLA和NanoTerasu。其中，散射与成像部显微动态成像团队负责开发利用同步辐射的X射线成像系统，并为想要获取X射线显微图像的研究人员提供技术支持。ORCA®-Quest qCMOS®相机被用作该X射线显微成像系统的探测器。
National Institute of Advanced Industrial Science and Technology (AIST)下属National Metrology Institute of Japan (NMIJ) 的Research Institute for Measurement and Analytical Instrumentation之中的Advanced Beam Measurement Group，正在致力于开发用于先进重离子放射治疗的α射线成像技术。这项研究使用ORCA®-Quest qCMOS®相机作为探测器。
光谱学
qCMOS相机具有极低的暗噪声等优异特性，弱信号检出能力出色。本实验结合使用 qCMOS相机与光栅光谱仪，并开发了新的软件同时控制这两种装置，用于检测针尖增强拉曼散射 (TERS)。为此，该团队测试了qCMOS相机对于拉曼或弱信号拉曼的检测能力，并探索了其他应用可能性。
在拉曼成像中，缩短测量时间是一个非常重要的因素。要实现这一点，需要在短时间内检测拉曼信号，这就要求相机具有高灵敏度和低噪声，以及多像素和高速度。我们测量了我们的光子数分辨相机 (qCMOS相机)的信噪比和光谱动态范围，并与EM-CCD相机进行比较，结果表明了qCMOS相机的优越性。
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