最近,对 iPS 细胞(诱导性多能干细胞)的研究在药物发现领域产生了巨大影响。目前,人 iPS 细胞 (hiPSC) 衍生出的各种特定类型的细胞(如心肌细胞和神经细胞)现已广泛上市销售,可以使用这些 hiPSC 源性细胞筛选化合物以进行药物发现。预计使用 hiPSC 源性细胞进行筛选,可提供更有效和更简便的方式,以评估基于细胞的测定中测试化合物的药理学和毒性作用。
滨松 FDSS 系列能够测量和分析 hiPSC 源性心肌细胞中的钙瞬态。
FDSS-GX 和 FDSS/μCELL 都可以提供 iPSC 心肌细胞钙瞬态所需的“加热器”、“高速记录”和“波形分析”选项。在处理 iPSC 时,“加热器”功能越来越重要,因为它在检测过程中的细胞状态和状况尤其会影响心肌细胞的起搏率。监测钙瞬态的另一个关键技术和功能是“高速记录”能力。使用我们的高灵敏度高速相机,最大采样率约为 100 Hz,最短间隔为 10 ms。我们还提供“波形分析”软件选项,用于分析所测量钙瞬态数据的峰值、跳动率、振幅、上升倾斜度、下降倾斜度 PWD 的数量。
作者 | 标题 | 来源 |
---|---|---|
Stephane Bedut、Christine Seminatore Nole、Veronique Lamamy、Sarah Caignard、Jean Albert Boutin、Olivier Nosjean、Jean-Philippe Stephan、Francis Coge | 在人 iPSC 源性心肌细胞中使用电压和钙敏感荧光探针进行高通量药物分析 | 美国生理学杂志 - 心脏和循环生理学 2016 年 5 月 3 日出版,卷号: DOI: 10.1152/ajpheart.00793.2015 |
Haoyu Zeng、Maria I. Roman、Edward Lis、Armando Lagrutta、Frederick Sannajust、SALAR,西点军校默克研究实验室的安全与探索性药理学部 (PA 19486, USA) | 使用 FDSS/μCell 成像平台对人诱导多能干细胞衍生的心肌细胞中的化合物进行临床前心脏电生理学安全性筛查 | 收稿日期:2016 年 2 月 29 日 修订日期:2016 年 5 月 6 日 接受日期:2016 年 5 月 18 日 2016 年 5 月 21 日在线提供 http://dx.doi.org/10.1016/j.vascn.2016.05.009 |
Dinesh Puppala、Leon P. Collis、Sunny Z. Sun、Vicniius Bonato、Xian Chen、Blake Anson、Mathew Pletcher、Bernard Fermini、Sandra J. Engle | 人诱导多能干细胞中的基因表达分析比较—衍生的心肌细胞和人类及猕猴心脏组织 | Toxicol. Sci. (2013) 131 (1):292-301. 首次在线发布日期:2012 年 9 月 14 日 |
应用 说明第 | 标题 |
---|---|
应用说明第 17 | FDSS 应用说明 17,Ca2+ 振荡在原代神经元培养中的使用:一种新的神经元疾病 HTS 模型 [0.3MB/PDF] |
应用说明第 22 | FDSS 应用说明 22,FDSS 系列的高速采集 [0.4MB/PDF] |
年份 | 标题 |
---|---|
2016 | 触发各种人类 iPSC 衍生的神经细胞类型的钙反应 Giorgia Salvagiotto,CDI - 富士公司,英国 |
2015 | Axiogenesis 正在扩大其关于人类 iPSC 衍生的细胞的产品组合 |
2015 | 通过 FDSS/μCell 中的电场刺激测量人类 iPSC 衍生的外周神经元中的 Ca2 反应 |
2015 | 使用滨松 FDSS/μCELL 对人类 iPSC 衍生的神经元进行电场刺激 (EFS) |
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