G 蛋白偶联受体 (GPCR) 的研究和筛选是从 FDSSⓇ 动态板成像仪高通量功能获益最多的领域。FDSS 可配备 96、384(FDSS/μCELL 和 FDSS-GX)和 1536 (FDSS-GX) 歧管移液器,以同时将配体/化合物添加到所有孔中,并对整个板成像,以便同时读取所有孔,从而在细胞反应发生时,孔间不会出现时间滞后。GPCR 在细胞信号转导中起着重要作用,许多靶向 GPCR 医疗药物已开发出来。应用(和资源)包括:
作者 | 标题 | 来源 |
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Le Poul E、Hisada S、Mizuguchi Y、Dupriez VJ、Burgeon E、Detheux M。 | 水母发光蛋白功能测定法在高通量筛选中的应用 | J Biomol Screen。2002 年 2 月;7(1):57-65。 |
Inbe H、Watanabe S、Miyawaki M、Tanabe E、Encinas JA。 | AMP 和腺苷的细胞表面受体 P2Y15 的鉴定和表征 | J Biol Chem。2004 年 5 月 7 日;279(19):19790-9。2004 年 3 月 4 日电子出版。 |
McNamara CR、Mandel-Brehm J、Bauista DM、Siemens J、Deranian KL、Zhao M、Hayward NJ、Chong JA、Julius D、Moran MM、Fanger CM。 | TRPA1 介导福尔马林引起的疼痛 | http://www.ncbi.nlm.nih.gov/pubmed/17686976 |
Cassutt KJ、Orsini MJ、Abousleiman M、Colone D、Tang W。 | 从均质钙测定屏幕识别非选择性命中 | J Biomol Screen。2007 年 3 月;12(2):285-7。2007 年 2 月 8 日电子出版。 |
Wise H、Abel PW、Cawkill D。 | 使用温度降低的细胞暂停来增强基于细胞的测定的灵活性 | J Biomol Screen。2009 年 7 月;14(6):716-22。2009 年 5 月 21 日电子出版。 |
Lin Tian、S. Andrews Hires、Tianyi Mao、Daniel Huber、M. Eugenia Chiappe、Sreekanth H. Chalasani、Leopoldo Petreanu、Jasper Akerboom、Sean A. McKinney、Eric R. Schreiter、Cornelia I. Bargmann、Vivek Jayaraman、Karel Svoboda、Loren L. Looger | 使用改进的 GCaMP 钙指示剂来对蠕虫、苍蝇和小鼠的神经活动成像 | Nature Methods 6,875 - 881 (2009),在线:2009 年 11 月 8 日 | doi:10.1038/nmeth.1398 |
Pantel J、Williams SY、Mi D、Sebag J、Corbin JD、Weaver CD、Cone RD。 | 使用实时 cAMP 测定法开发黑皮质素 4 受体信号变构调节剂的高通量筛选 | Eur J Pharmacol。2011 年 6 月 11 日;660(1):139-47。2011 年 2 月 4 日电子出版。 |
Jasper Akerboom、Tsai-Wen Chen、Trevor J. Wardill、Lin Tian、Jonathan S. Marvin、Sevinç Mutlu、Nicole Carreras Calderón、Federico Esposti、Bart G. Borghuis、Xiaonan Richard Sun、Andrew Gordus、Michael B. Orger、Ruben Portugues、Florian Engert、John J. Macklin、Alessandro Filosa、Aman Aggarwal、Rex A. Kerr、Ryousuke Takagi、Sebastian Kracun、Eiji Shigetomi、Baljit S. Khakh、Herwig Baier、Leon Lagnado、Samuel S.-H. Wang、Cornelia I. Bargmann、Bruce E. Kimmel、Vivek Jayaraman、Karel Svoboda、Douglas S. Kim、Eric R. Schreiter、Loren L. Looger | 优化用于神经活动成像的 GCaMP 钙指示剂 | 本文通过 J Neurosci Open Choice 选项在线免费提供。2012 年 8 月 1 日 |
Olaposi I. Omotuyi、Jun Nagai、Hiroshi Ueda | 在受体激活期间,Lys39 溶血磷脂羰基氧相互作用将 LPA1 N 末端帽锁定到正构位点并结合 Arg124 | 科学报告 5,文章编号:13343 (2015)doi:10.1038/srep13343 |
应用 说明第 | 标题 |
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FDSS 应用说明第 14 | FDSS 用途说明 14,使用 FDSS6000 评估新型细胞内钙荧光试剂 [0.4MB/PDF] |
FDSS 应用说明第 20 | FDSS 用途说明 20,用于药物发现的新工具:使用 FDSS/μCell 对原代细胞中的 Ca2+ 和 cAMP 信号传导进行多重监测。 [0.4MB/PDF] |
FDSS 应用说明第 23 | FDSS 应用说明 23,使用 FDSS/μCELL 成像读板机进行 BRET1 测定 [0.13MB/PDF] |
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