FDSS® 是荧光和发光领域的“添加和读取”动态板成像仪,适用于读取那些反应、钙通量、流动、膜电位变化的活细胞的动力学,这种变化非常快,需要监测其动力学特性。GPCR 和离子通道以及转运蛋白是 FDSS 动态板成像仪最常用的应用领域,用于监测活细胞的钙瞬态和膜电位变化。
然而,在酶领域也做了一些创造性的工作,FDSS 现在并不限于活细胞。通常,酶测定不需要“添加和读取”动态板成像仪,因为其反应进行得非常缓慢,因此每 30 分钟或每小时一个采样点就足够了。但有些酶反应非常快,这需要我们的“添加和读取”动态板成像仪。
在反应的初始速度阶段测量酶活性非常重要。然而,过去对检测信号的持续监测并不总是可行的。FDSS 为 HTS 环境提供了解决方案,尤其是快速反应。
这里的示例均由当时的高端系统 FDSS7000EX 或 FDSS6000 完成。FDSS-GX 继承了所有光学设计,并与 FDSS7000EX 和 FDSS6000 完全兼容。这些测定法正在利用 FDSS-GX 中提供的紫外线激发。
作者 | 标题 | 来源 |
---|---|---|
Mori T、Itami S、Yanagi T、Tatara Y、Takamiya M、Uchida T。 | 使用实时荧光监测系统对脯氨酰异构酶抑制剂进行高通量筛选 | J Biomol Screen。2009 年 4 月;14(4):419-24。 |
Erica VanderPorten、Lauren Frick、Rebecca Turincio、Peter Thana、William LaMarr、Yichin Liu | 用于筛选和表征新型乳酸脱氢酶抑制剂的无标记高通量测定 | 2013 年 10 月 15 日,第 2 期,第 441 卷,第 115–122 页 |
应用 说明第 | 标题 |
---|---|
FDSS 应用说明第 19 | FDSS 用途说明 19,快速动力学酶测定脯氨酰异构酶 (Pin1) 抑制剂的高通量筛选 [0.2MB/PDF] |
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