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ORCA®-Flash4.0 V3 数字 CMOS 相机
C13440-20CU
我们改进了相机技术，以便您推进科学研究
凭借我们在高性能科学相机和高级成像用途方面的丰富经验，滨松推出了新的 ORCA-Flash4.0 V3。无论是采集精美科学图像，还是需要检测、定量和速度的实验等各种用途，这款相机均能应付自如。ORCA-Flash4.0 V3 具有板载 FPGA 处理功能，可实现智能数据缩减、高度精细的相机内、像素级校准、更高的 USB3.0 帧速率、针对性的创新触发功能、获得专利的光片读出模式和单独的相机噪声特性化，是成像的精密仪器。
ORCA是日本滨松光子学株式会社，及其全球子公司（欧洲联盟、日本、英国、美国）注册使用的商标。
特点
- 校准定量精度
- 自定义数据控制的灵活性
- 用于高级成像的专利工具：光片读出模式
- 关注相关数据
- 强大的同步触发
光片读出模式 [专利]
“光片读出模式”是滨松 sCMOS 相机的独特专利功能，可提高光片显微镜中的信噪比。
有关光片读出模式原理和特点的更多信息，请参阅下面的详细信息。
视频库
出版物
- ORCA-Flash4.0
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作者 标题 来源 Xuanze Chen, Zhiping Zeng, Rongqin Li, Boxin Xue, Peng Xi, Yujie Sun Superior performance with sCMOS over EMCCD in super-resolution optical fluctuation imaging 生物医学光学杂志 Luchang Li, Mengting Li, Zhaoning Zhang, Zhen-Li Huang Assessing low-light cameras with photon transfer curve method 创新光学健康科学杂志 S Hayashi, Y Okada Ultrafast superresolution fluorescence imaging with spinning disk confocal microscope optics 细胞分子生物学 Matthias Mehling, Tino Frank, Cem Albayrak, Sava? Tay Real-time tracking, retrieval and gene expression analysis of migrating human T cells 芯片实验室 Ying S. Hu, Maxwell Zimmerley, Yu Li, Robin Watters, and Hu Cang Single-Molecule Super-Resolution Light-Sheet Microscopy 化学物理化学 Peter W Winter and Hari Shroff Faster fluorescence microscopy: advances in high speed biological imaging 化学生物学最新观点 Ronny Forster, Hui-Wen Lu-Walther, Aurelie Jost, Martin Kielhorn, Kai Wicker, and Rainer Heintzmann Simple structured illumination microscope setup with high acquisition speed by using a spatial light modulator 光学快报 Jeffrey P. Nguyen, Frederick B. Shipley, Ashley N. Linder, George S. Plummer, Joshua W. Shaevitz, and Andrew M. Leifer Whole-brain calcium imaging with cellular resolution in freely behaving C. elegans 神经元和认知 Zong W, Zhao J, Chen X, Lin Y, Ren H, Zhang Y, Fan M, Zhou Z, Cheng H, Sun Y, Chen L. Large-field high-resolution two-photon digital scanned light-sheet microscopy 细胞研究，2014 年 9 月 26 日，doi: 10.1038/cr.2014.124。[印刷前电子出版物] Vladimirov N, Mu Y, Kawashima T, Bennett DV, Yang CT, Looger LL, Keller PJ, Freeman J, Ahrens MB. Light-sheet functional imaging in fictively behaving zebrafish Nat Methods。2014 年 7 月 27 日，doi: 10.1038/nmeth.3040。[印刷前电子出版物] Mickoleit M, Schmid B, Weber M, Fahrbach FO, Hombach S, Reischauer S, Huisken J. High-resolution reconstruction of the beating zebrafish heart Nat Methods。2014 年 7 月 20 日，doi: 10.1038/nmeth.3037。[印刷前电子出版物] Vettenburg T, Dalgarno HI, Nylk J, Coll-Lladó C, Ferrier DE, Čižmár T, Gunn-Moore FJ, Dholakia K. Light-sheet microscopy using an Airy beam Nat Methods。2014 年 5 月，11(5):541-4. doi: 10.1038/nmeth.2922。2014 年 4 月 6 日电子出版。 Juette MF、Terry DS1、Wasserman MR、Zhou Z、Altman RB、Zheng Q、Blanchard SC。 The bright future of single-molecule fluorescence imaging 化学生物学最新观点 2014 年 6 月 20 日；20C:103-111. doi: 10.1016/j.cbpa.2014.05.010。 Menon M, Askinazi OL, Schafer DA. Dynamin2 organises lamellipodial actin networks to orchestrate lamellar actomyosin PLoS One。2014 年 4 月 7 日，9(4):e94330. doi: 10.1371/journal.pone.0094330. eCollection 2014。 Gualda EJ, Vale T, Almada P, Feijó JA, Martins GG, Moreno N. OpenSpinMicroscopy: an open-source integrated microscopy platform Nat Methods。2013 年 7 月，10(7):599-600. doi: 10.1038/nmeth.2508。2013 年 6 月 9 日电子出版。 Fahrbach FO, Voigt FF, Schmid B, Helmchen F, Huisken J. Rapid 3D light-sheet microscopy with a tunable lens 光学快报。2013 年 9 月 9 日，21(18):21010-26. doi: 10.1364/OE.21.021010。 Fullerton S, Bennett K, Toda E, Takahashi T. Optimization of precision localization microscopy using CMOS camera technology 国际光电工程学会 SPIE 8228, Single Molecule Spectroscopy and Superresolution Imaging V, 82280T （2012 年 2 月 9 日）; doi:10.1117/12.906336; http://dx.doi.org/10.1117/12.906336 Fullerton S, Bennett K, Toda E, Takahashi T. Camera simulation engine enables efficient system optimization for super-resolution imaging 国际光电工程学会 SPIE 8228, Single Molecule Spectroscopy and Superresolution Imaging V, 822811 (February 9, 2012); doi:10.1117/12.906346; http://dx.doi.org/10.1117/12.906346 Singh AP, Krieger JW, Buchholz J, Charbon E, Langowski J, Wohland T. The performance of 2D array detectors for light sheet based fluorescence correlation spectroscopy. 光学快报。2013 年 4 月 8 日，21(7):8652-68。 Ahrens MB, Keller PJ. Whole-brain functional imaging at cellular resolution using light-sheet microscopy. Nat Methods。2013 年 3 月 18 日，doi: 10.1038/nmeth.2434。 Long F, Zeng S, Huang ZL. Localization-based super-resolution microscopy with an sCMOS camera part II: experimentalmethodology for comparing sCMOS with EMCCD cameras. 光学快报。2012 年 7 月 30 日，20(16):17741-59。 Baumgart E, Kubitscheck U. Scanned light sheet microscopy with confocal slit detection. 光学快报。2012 年 9 月 10 日；20(19):21805-14。 Takao D, Nemoto T, Abe T, Kiyonari H, Kajiura-Kobayashi H, Shiratori H, Nonaka S. Asymmetric distribution of dynamic calcium signals in the node of mouse embryo during left-right axis formation. 发育生物学 2013 年 4 月 1 日；376(1):23-30。 Huang F, Hartwich TM, Rivera-Molina FE, Lin Y, Duim WC, Long JJ, Uchil PD, Myers JR, Baird MA, Mothes W, Davidson MW, Toomre D, Bewersdorf J. Video-rate nanoscopy using sCMOS camera-specific single-molecule localization algorithms. Nat Methods。2013 年 5 月 26 日，doi: 10.1038/nmeth.2488。 Ma H, Kawai H, Toda E, Zeng, S, Huang ZL Localization-based super-resolution microscopy with an sCMOS camera part III: camera embedded data processing significantly reduces the challenges of massive data handling 光学快报，2013 年第 11 期，第 38 卷，第 1769-1771 页 Baumgart E., Kaminski T. Scanned LSFM with Confocal Detection. 成像和显微镜，2013 年第 1 期，第 15 卷。 Xu D, Jiang T, Li A, Hu B, Feng Z, Gong H, Zeng S, Luo Q. Fast optical sectioning obtained by structured illumination microscopy using a digital mirror device. J Biomed Opt. 2013 年 6 月，18(6):60503. doi: 10.1117/1.JBO.18.6.060503.
PC 建议
随着 ORCA-Flash4.0 的推出，用户现在能够以每秒 100 帧的速度将 400 万像素的图像流式传输到他们的计算机。通过使用 ORCA-Flash4.0 PC 建议列出的指南，可以满足计算机对高数据率的建议。
软件
详细参数
| 类型编号 | C13440-20CU |
|---|---|
| 成像设备 | sCMOS |
| 有效像素数 | 2048 (H)×2048 (V) |
| 细胞大小 | 6.5 μm×6.5 μm |
| 有效面积 | 13.312 mm×13.312 mm |
| 最大阱容 | 30,000 个电子（典型值）* |
| 读出速度 | 100 帧/秒（全分辨率、标准扫描、Camera Link）* 40 帧/秒（全分辨率、标准扫描、USB 3.0、16 位）* 53 帧/秒（全分辨率、标准扫描、USB 3.0、12 位）* 80 帧/秒（全分辨率、标准扫描、USB 3.0、8 位）* |
| 读出噪声 | 标准扫描（100 帧/秒，典型值）：1.6 个电子 rms（1.0 电子中值） 慢速扫描（30 帧/秒，典型值）：1.4 个电子 rms（0.8 电子中值） |
| 冷却方法 | 珀耳帖制冷 |
| 冷却温度 | 强制风冷（环境温度 +20 ̊C）：-10 ̊C 水冷 (+20 ̊C)：-10 ̊C 水冷 (+15 ̊C)：-30 ̊C |
| 暗电流 | 0.06 个电子/像素/秒（风冷至 -10 ̊C）（典型值） 0.06 个电子/像素/秒（水冷至 -10 ̊C）（典型值） 0.006 个电子/像素/秒（水冷至 -30 ̊C）（典型值） |
| 动态范围 | 37,000:1（典型值） |
| 接口 | 相机链接/USB 3.0 |
| AD 转换器 | 16 位/12 位/8 位 |
| 透镜接口 | C 型接口 |
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