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ORCA®-Fusion BT 数字 CMOS 相机
C15440-20UP
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ORCA-Fusion BT 相机是科学 CMOS (sCMOS) 性能的巅峰之作。规格不折不扣：超低读出噪声、媲美 CCD 的均匀性、快速帧速率和背照式实现的高 QE。但 ORCA-Fusion BT 的优点在于，这种卓越的光子检测和采集组合能够带来什么功能。查看最暗淡的信号，从最少的光子中获取视觉上令人惊叹的高 S/N 图像，捕获以前未解决的时间事件，并自信地执行计算方法。ORCA-Fusion BT 相机使实验化繁为简，使以前不可能进行的实验变得可能。
ORCA是日本滨松光子学株式会社，及其全球子公司（欧洲联盟、日本、英国、美国）注册使用的商标。
ORCA-Fusion BT 对比其他 BT CMOS 相机的优势
行业领先的独家 BT sCMOS 性能，具有值得信赖的滨松质量和可靠性
- 极低读出噪声
- 高 QE，宽波长范围
- 媲美 CCD 的均匀性；最低高读出噪声像素
- 高动态范围
- 3 种读出速度
- 光片读出模式
- 530 万像素
- USB 3.0 和高速 CoaXPress 接口
ORCA-Fusion BT 对比 EM-CCD 相机的优势
一款实用且功能强大的替代产品，可完美替代昂贵的 EM-CCD，具有出色的灵敏度、速度和通用性
- 分辨率
- 像素合并通用性
- 无放大或过剩噪声
- 高动态范围
- 大视野
用途
- 宽视野荧光显微镜
- 延时成像
- 转盘共焦显微镜
- 光片显微镜
- 超高分辨率精确定位
- 基因编码电压成像
- 光遗传学
- 单分子跟踪
- 计算成像
- 量子计算
通过低光激发进行延时活细胞成像
样品：H9c2，物镜：Apo TIRF 60XC (NA1.49)，ND：8，间隔：15 秒
细胞核
细胞核：
HCS NuclearMask 染色剂/曝光 20 ms
线粒体
线粒体：
MitoTracker/曝光 500 ms
薄膜
薄膜：
CellMask 血浆膜染色剂/曝光 500 ms
光片读出模式 [专利]
“光片读出模式”是滨松 sCMOS 相机的独特专利功能，可提高光片显微镜中的信噪比。
有关光片读出模式原理和特点的更多信息，请参阅下面的详细信息。
出版物
- ORCA-Fusion BT
-
作者 标题 来源 Pratish Thakore, Michael G Alvarado, Sher Ali, Amreen Mughal, Paulo W Pires, Evan Yamasaki, Harry AT Pritchard, Brant E Isakson, Cam Ha T Tran, Scott Earley Brain endothelial cell TRPA1 channels initiate neurovascular coupling 电子生活，10, e63040 (2021). Lexy von Diezmann and Ofer Rog Single-Molecule Tracking of Chromatin-Associated Proteins in the C. elegans Gonad J. Phys. Chem. B 2021, 125, 23, 6162–6170 Kei Yamamoto, Haruko Miura, Motohiko Ishida, Yusuke Mii, Noriyuki Kinoshita, Shinji Takada, Naoto Ueno, Satoshi Sawai, Yohei Kondo & Kazuhiro Aoki Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis 自然通讯，12, 7145 (2021)。 Mbaye Diouf, Mitchell Harling, Murat Yessenov, Layton A. Hall, Ayman F. Abouraddy, and Kimani C. Toussaint, Space-time vector light sheets 光学 快报，29, 37225-37233 (2021) Oliver Klatt, Daniele Repetto, Johannes Brockhaus, Carsten Reissner, Abderazzaq El khallouqi, Astrid Rohlmann, Martin Heine, Markus Missler, Endogenous β-neurexins on axons and within synapses show regulated dynamic behavior 细胞报告，2021 年第 11 期，第 35 卷，109266， Satoshi Okada, Goro Doi, Shitomi Nakagawa, Emiko Kusumoto, Takashi Ito Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae G3 基因|基因组|遗传学，2021 年 12 月，第 12 期，第 11 卷，jkab304
PC 建议
随着 ORCA-Fusion BT 的推出，用户现在能够以每秒 89 帧的速度将 500 万像素的图像流式传输到他们的计算机。通过使用 ORCA-Fusion BT PC 建议列出的指南，可以满足计算机对高数据率的建议。
软件
详细参数
| 产品编号 | C15440-20UP |
|---|---|
| 成像设备 | 科学 CMOS 图像传感器 |
| 有效像素数 | 2304 (H) × 2304 (V) |
| 像素尺寸 | 6.5 μm × 6.5 μm |
| 有效面积 | 14.976 mm (H) × 14.976 mm (V) |
| 最大阱容 | 15,000 个电子 *1 |
| 读出速度 | 快速扫描，全分辨率，CoaXPress：89.1 帧/秒 快速扫描，全分辨率，USB 3.0：31.6 帧/秒（16 位），42.2 帧/秒（12 位），63.3 帧/秒（8 位） 标准扫描，全分辨率，CoaXPress：23.2 帧/秒 标准扫描，全分辨率，USB 3.0：23.2 帧/秒 超静音扫描，全分辨率，CoaXPress：5.42 帧/秒 超静音扫描，全分辨率，USB 3.0：5.42 帧/秒 |
| 读出噪声 | 快速扫描：1.6 个电子 rms *1 标准扫描：1.0 个电子 rms *1 超静音扫描：0.7 个电子 rms *1 |
| 曝光时间 | 快速扫描：17 μs 至 10 s 标准扫描：65 μs 至 10 s 超静音扫描：280 μs 至 10 s |
| 冷却方法 | 珀耳帖制冷 |
| 冷却温度 | 强制风冷（环境温度：+25 ̊C）：−8 ˚C 水冷（水温：+25 ˚C）：−8 ˚C 水冷（最大制冷（环境温度：+20 ˚C，水温：+20 ˚C））：低于 −15 ˚C |
| 暗电流 | 1.0 个电子/像素/秒（冷却温度：-8 ˚C，环境温度：+25 ˚C）*1*3 1.0 个电子/像素/秒（冷却温度：-8 ˚C，水温：+25 ˚C）*1*3 0.7 个电子/像素/秒（冷却温度：低于 -15 ̊C，环境温度：+20 ˚C，水温：+20 ̊C）*1*3 |
| 动态范围 | 21 400:1 *1*2*4 |
| 外部触发模式 | 边缘、电平、同步读出、开始、全局复位边缘、全局复位电平、可编程 |
| 外部触发信号路由 | SMA |
| 外部触发延迟功能 | 0 μs 至 10 s（1 μs 步长） |
| 触发输出 | 全局曝光定时输出、触发就绪输出、低输出、高输出、3 个可编程定时输出 |
| 外部信号输出路由 | SMA |
| 接口 | CoaXPress（双 CXP-6）和 USB 3.0 Super Speed *5 |
| AD 转换器 | 16 位、12 位、8 位 |
| 透镜接口 | C 型接口（标准），F 型接口 (C15440-20UP01) |
| 电源 | 交流 100 V 至交流 240 V，50 Hz/60 Hz |
| 用电功耗 | 约 155 VA |
| 操作环境温度 | 0 ̊C 至 +40 ̊C |
| 存储环境温度 | -10 ℃ 到 +50 ℃ |
| 操作环境湿度 | 30% 至 80%（无冷凝） |
| 存储环境湿度 | 90% 或更低（无冷凝） |
*1 典型值
*2 根据最大阱容和 RMS 读取噪声的比率计算
*3 暗电流取决于冷却温度
*4 在超安静扫描模式下
*5 相当于 USB 3.1 Gen 1 (SuperSpeed USB 5 Gbps)
光谱灵敏度特性
尺寸
说明手册
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