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ORCA®-Fire 数字 CMOS 相机
C16240-20UP
启迪发现的要素
ORCA-Fire 智能地集成了高性能、薄型背照式科学 CMOS (sCMOS) 相机的所有基本要素。该相机的卓越性能源于滨松对低噪声和高量子效率 sCMOS 技术的不懈追求。借助 ORCA-Fire，在实现高灵敏度的同时，还可获得出色的分辨率和超快的速度。当科研需要高吞吐量，但样品只能提供几个光子时，ORCA-Fire 将大放异彩。
ORCA-Fire 会激发您的下一个发现吗？
ORCA是日本滨松光子学株式会社，及其全球子公司（欧洲联盟、日本、英国、美国）注册使用的商标。
规格亮点
低噪声
1.0 个电子 rms （115 帧/秒）
高 QE
86% @460 nm（背照式 CMOS）
高分辨率
4432 (H) × 2368 (V)（像素尺寸 4.6 μm）
高速
115 帧/秒（@4432(H)×2368(V) 1050 万像素）
大视野
20.4 mm × 10.9 mm（对角线 23.114 mm）
高动态范围
1：20 000（最大阱容 20 000 个电子）
小像素，大分辨率
优化您的光学器件，使分辨率最大化
低放大率成像 (<40×) 具有大视野的优势，这对于高通量应用至关重要。要获取具有最大信息量的低放大率图像，成像系统必须通过将像素尺寸与奈奎斯特级或更高的采样率相匹配来实现高分辨率。 ORCA-Fire 的像素尺寸非常适合大多数 40 倍或更低倍率的物镜（见下图）。 ORCA-Fire 具有高空间分辨率、大像素阵列和快速读出速度，像素吞吐量比最快的 4.2 MP 6.5 μm sCMOS 相机还高出 2.9 倍。
根据物镜放大率和 NA，合适的传感器像素尺寸示例
| 放大率 | NA | δ (μm) | Δ (μm) | 合适的像素尺寸 (μm) |
|---|---|---|---|---|
| 4 | 0.16 | 2.10 | 8.4 | 4.2 |
| 10 | 0.4 | 0.84 | 8.4 | 4.2 |
| 20 | 0.8 | 0.42 | 8.4 | 4.2 |
| 40 | 1.4 | 0.24 | 9.6 | 4.8 |
| 40 | 0.95 | 0.35 | 14.1 | 7.1 |
| 60 | 1.42 | 0.24 | 14.2 | 7.1 |
| 100 | 1.5 | 0.22 | 22.4 | 11.2 |
* 瑞利准则 (δ) = 0.61λ / NA
* 波长 (λ) = 550 nm
* Δ = δ × 物镜放大率
不同像素尺寸的图像质量比较
高 QE 和低噪声
实现高灵敏度，无需任何牺牲
ORCA-Fire 采用先进的薄型背照式技术与微透镜，以实现高量子效率。结合 1.0 e- rms 的读出噪声，ORCA-Fire 延续了滨松提供的相机在所有光量下都具有最高灵敏度的趋势。
深沟槽结构和薄型背照
高 QE 是高灵敏度成像的基本预期和关键组成部分。 通过薄型背照式传感器实现高 QE 似乎很简单，但薄型背照式传感器设计中存在可能影响图像质量的微妙之处。 在传统的背照式探测器中，由于硅的有源区域内的像素分离不佳，像素之间会出现串扰，从而影响分辨率，而与像素尺寸无关。 我们的工程师在 ORCA-Fire 中实现了深度沟槽像素结构，可防止像素串扰并提高分辨率。
什么是沟槽结构？
选择您的速度
每台 ORCA-Fire 都具有 CoaXPress 和 USB 连接
读出速度（帧/秒）
| 读出模式 | 区域读出模式 | |||
|---|---|---|---|---|
| 扫描模式 | 标准扫描 | |||
| X（像素） | Y（像素） | CoaXPress | USB3.1 Gen I （16 位） |
USB3.1 Gen I （8 位） |
| 4432 | 2368 | 115 | 15.7 | 31.5 |
| 4432 | 2304 | 118 | 16.2 | 32.4 |
| 4432 | 2048 | 132 | 18.2 | 36.5 |
| 4432 | 1024 | 264 | 36.4 | 72.8 |
| 4432 | 512 | 524 | 72.3 | 144 |
| 4432 | 256 | 1020 | 143 | 286 |
| 4432 | 128 | 1980 | 279 | 558 |
| 4432 | 8 | 15 200 | 2360 | 5260 |
| 4432 | 4 | 19 500 | 3960 | 7200 |
2×2 像素合并时的读出速度（帧/秒）
| 读出模式 | 区域读出模式 | |||
|---|---|---|---|---|
| 扫描模式 | 标准扫描 | |||
| X（像素） | Y（像素） | CoaXPress | USB3.1 Gen I （16 位） |
USB3.1 Gen I （8 位） |
| 2216 | 1184 | 115 | 63.1 | 115 |
| 2216 | 1152 | 118 | 64.9 | 118 |
| 2216 | 1024 | 132 | 73 | 132 |
| 2216 | 512 | 264 | 145 | 264 |
| 2216 | 256 | 524 | 289 | 524 |
| 2216 | 128 | 1020 | 572 | 1020 |
| 2216 | 64 | 1980 | 1110 | 1980 |
| 2216 | 4 | 15 200 | 10 500 | 15 200 |
| 2216 | 2 | 19 500 | 13 600 | 19 500 |
百万像素/秒
扩大您的视野
视野比较
ORCA-Fire 具有 4432 (H) × 2368 (V) 个像素，可有效利用 22 mm 显微镜视野。
光片：专为专家打造
光片读出模式可减少散射光效应
研究人员日益转向荧光光片显微镜来研究活细胞和生物体中的生物过程，并捕获透明组织的惊人的 3D 分辨率图像。 光片显微镜有许多种类，但通常使用“光片”正交照射样品。 然后针对整个样品扫描此光片，获得可以重新组合成完整 3D 渲染的光学剖面图像。ORCA-Fire 采用滨松的专利光片读出模式。 在此模式下，光片与传感器的读出同步，从而减少散射光的影响，并有效提高图像质量和信噪比。
光片读出模式帧速率（帧/秒）
| 读出模式 | 光片读出模式 | |||
|---|---|---|---|---|
| 扫描模式 | 标准扫描 | |||
| X（像素） | Y（像素） | CoaXPress | USB3.1 Gen I （16 位） |
USB3.1 Gen I （8 位） |
| 4432 | 2368 | 114 | 15.7 | 31.5 |
| 4432 | 2304 | 117 | 16.2 | 32.4 |
| 4432 | 2048 | 132 | 18.2 | 36.5 |
| 4432 | 1024 | 263 | 36.4 | 72.8 |
| 4432 | 512 | 518 | 72.3 | 144 |
| 4432 | 256 | 1000 | 143 | 286 |
| 4432 | 128 | 1900 | 279 | 558 |
| 4432 | 8 | 11 400 | 2630 | 5260 |
| 4432 | 4 | 13 600 | 3690 | 7200 |
sCMOS 光片读出模式比较
| 有效像素数 (H)×(V) | 读出速度（帧/秒） | ||
|---|---|---|---|
ORCA-Fire (CoaXPress) |
ORCA-Fusion |
ORCA-Flash4.0 V3 |
|
| 4432 × 2368 | 114 | - | - |
| 2304 × 2304 | 117 | 88.9 | - |
| 2048 × 2048 | 132 | 100 | 49 |
| 1024 × 1024 | 263 | 199 | 99 |
| 512 × 512 | 518 | 396 | 196 |
| 256 × 256 | 1000 | 784 | 384 |
| 128 × 128 | 1900 | 1540 | 738 |
接口：CoaXPress/Camera Link
图像捕获模式：内部同步模式
在光片读出模式下，ORCA-Fire 每秒提供的像素数比最快的低噪声 sCMOS 相机还 高出 2.5 倍 。
光片：在任何方向同步
双向读出消除帧间延迟
在 ORCA-Fire 中，光片读出有四种不同的操作模式：前向、后向、双向和反向双向。在前向模式下，读出从传感器的顶部开始，一直进行到底部。在后向模式下，读出从底部开始，在顶部结束。双向模式在开始的第一帧采用前向读出，然后在下一帧切换到后向读出，依此类推，以交替的方式逐帧继续。顾名思义，反向双向模式从底部向顶部开始，第一帧采用后向读出，然后在下一帧切换到从顶部到底部的读出方式，依此类推。两种双向模式均已实施，以避免在下一帧需要延迟时间才能将光片返回到传感器的顶部或底部。
PC 建议
随着 ORCA-Fire 的推出，用户现在能够以每秒 115 帧的速度将 1000 万像素的图像流式传输到他们的计算机。通过使用 ORCA-Fire PC 建议列出的指南，可以满足计算机对高数据率的建议。
软件
详细参数
| 产品编号 | C16240-20UP |
|---|---|
| 成像设备 | 科学 CMOS 图像传感器 |
| 有效像素数 | 4432 (H)×2368 (V) |
| 像素尺寸 | 4.6 μm×4.6 μm |
| 有效面积 | 20.387 mm×10.892 mm |
| 最大阱容 | 20 000 个电子（典型值） |
| 读出速度*1 | 全分辨率，CoaXPress：115 帧/秒 全分辨率，USB 3.1：15.7 帧/秒 垂直 4 线，CoaXPress：19 500 帧/秒 垂直 4 线，USB 3.1：3690 帧/秒 |
| 读出噪声 | 1.0 个电子 (rms)，0.9 个电子（中位数） |
| 量子效率 | 86%（峰值 QE）（典型值） |
| 曝光时间 | 7.309 μs 至 10 s（7.309 μs 步长） |
| 冷却方法 | 珀耳帖制冷 |
| 冷却温度 | 强制风冷（环境温度： +25 ̊C）+ 20°C |
| 暗电流 | 0.6 个电子/像素/秒（典型值） |
| 动态范围 *2 | 20 000:1 (rms)，22 000 : 1（中位数） |
| 传感器模式 | 区域读出，光片读出 |
| 外部触发功能 | 区域读出模式：边缘触发、全局复位边缘触发、电平触发、全局复位电平触发、同步读出触发、启动触发 光片读出模式：边缘触发、启动触发 |
| 外部触发信号 | 外部输入 (SMA) |
| 外部触发电平 | TTL/3.3 V LVCMOS 电平 |
| 外部触发延迟功能 | 0 μs 至 10 s（1 μs 步长） |
| 外部输出信号 | 全局曝光定时输出、任意行曝光定时输出、触发就绪输出、可编程定时输出、高输出、低输出 |
| 外部输出电平 | 3.3 V LVCMOS 电平 |
| 接口 | CoaXPress (Quad CXP-6) / USB 3.1 Gen 1 |
| AD 转换器 | 16 位、8 位 |
| 透镜接口 | C 型接口 |
| 电源 | AC 100 V 至交流 240 V，50 Hz/60 Hz，2.5 A |
| 用电功耗 | 100 VA |
| 操作环境温度 | 0 ̊C 至 +40 ̊C |
| 存储环境温度 | -10 ℃ 到 +50 ℃ |
| 操作环境湿度 | 30% 到 80% 或更低（无雾气现象） |
| 存储环境湿度 | 90% 或更低（无雾气现象） |
※1 使用 DCAM-API 的帧捆绑函数
※2 根据最大阱容和读出噪声之比计算
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