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ORCA®-Fusion 数字 CMOS 相机
C14440-20UP
ORCA-Fusion 由传感器向上构建，平衡了相机功能的复杂细微差别，可在所有光量，尤其是在恶劣的弱光条件下，提供漂亮的图像和强大的数据。ORCA-Fusion 极低且高度均匀的读取噪声意味着，如果在默认情况下或者根据实验设计，样品发射的光子数甚至只有少数，则它们不会在噪声中丢失，而是被检测到并可靠地量化。毕竟，当您想听到低语时，最好是在安静的地方。
ORCA是日本滨松光子学株式会社，及其全球子公司（欧洲联盟、日本、英国、美国）注册使用的商标。
特点
- 高分辨率：2304 × 2304（530 万像素）
- 高速：100 帧/秒（2304 × 2048 ROI 时）
- 动态范围：21,400:1
- 像素尺寸：6.5 μm × 6.5 μm
- 读取噪声：0.7 个电子 rms（超静音扫描）
- Prnu：0.06%（@ 7500 个电子）
- DSNU：0.06 个电子
- 峰值 QE：80 %
- 用于高级成像的专利工具：光片读出模式
光片读出模式 [专利]
“光片读出模式”是滨松 sCMOS 相机的独特专利功能，可提高光片显微镜中的信噪比。
有关光片读出模式原理和特点的更多信息，请参阅下面的详细信息。
出版物
- ORCA-Fusion
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作者 标题 来源 Piatkevich, K.D., Bensussen, S., Tseng, Ha. et al. Population imaging of neural activity in awake behaving mice 自然，574，413–417 (2019)。 Silvia Benito-Kwiecinski, Stefano L. Giandomenico, Magdalena Sutcliffe, Erlend S. Riis, Paula Freire-Pritchett, Iva Kelava, Stephanie Wunderlich, Ulrich Martin, Gregory A. Wray, Kate McDole, Madeline A. Lancaster An early cell shape transition drives evolutionary expansion of the human forebrain 细胞，2021 年第 8 期，第 184 卷，第 2084-2102 页.e19， Madhav Mantri, Gaetano J. Scuderi, Roozbeh Abedini-Nassab, Michael F. Z. Wang, David McKellar, Hao Shi, Benjamin Grodner, Jonathan T. Butcher & Iwijn De Vlaminck Spatiotemporal single-cell RNA sequencing of developing chicken hearts identifies interplay between cellular differentiation and morphogenesis 自然通讯，12, 1771 (2021)。 Kiryl D. Piatkevich, Seth Bensussen, Hua-an Tseng, Sanaya N. Shroff, Violeta Gisselle Lopez-Huerta, Demian Park, Erica E. Jung, Or A. Shemesh, Christoph Straub, Howard J. Gritton, Michael F. Romano, Emma Costa, Bernardo L. Sabatini, Zhanyan Fu, Edward S. Boyden & Xue Han Volumetric live cell imaging with three-dimensional parallelized RESOLFT microscopy 自然生物技术 39， 609–618 (2021)。 Xinyu Wang, Chong Liu, Siwei Zhang, Huiwen Yan, Liwen Zhang, Amin Jiang, Yong Liu, Yun Feng, Di Li, Yuting Guo, Xinyao Hu, Yajing Lin, Pengcheng Bu, Dong Li, N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles 发育细胞，2021 年第 5 期，第 56 卷，第 702-715 页.e8， Emily C. Wheeler, Anthony Q. Vu, Jaclyn M. Einstein, Matthew DiSalvo, Noorsher Ahmed, Eric L. Van Nostrand, Alexander A. Shishkin, Wenhao Jin, Nancy L. Allbritton & Gene W. Yeo Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors 自然方法，2020年，第 17卷，第636–642页 Scott M. Emrich, Ryan E. Yoast, Ping Xin, Vikas Arige, Larry E. Wagner, Nadine Hempel, Donald L. Gill, James Sneyd, David I. Yule, Mohamed Trebak, Omnitemporal choreographies of all five STIM/Orai and IP3Rs underlie the complexity of mammalian Ca2+ signaling 细胞报告，2021 年第 9 期，第 34 卷，108760， Tomomi Suzuki, Tetsuo Mioka, Kazuma Tanaka & Akira Nagatani An optogenetic system to control membrane phospholipid asymmetry through flippase activation in budding yeast 科学报告 10，12474（2020 年）。 Nicholas S. Groves,Merissa M. Bruns and Schuyler B. van Engelenburg A Quantitative Live-Cell Superresolution Imaging Framework for Measuring the Mobility of Single Molecules at Sites of Virus Assembly 病原体，2020 年，9(11)，972 Pierre Stömmer, Henrik Kiefer, Enzo Kopperger, Maximilian N. Honemann, Massimo Kube, Friedrich C. Simmel, Roland R. Netz & Hendrik Dietz A synthetic tubular molecular transport system 自然通讯，12，4393 (2021)。 Hugh Sparks, Lucas Dent, Chris Bakal, Axel Behrens, Guillaume Salbreux, and Chris Dunsby Dual-view oblique plane microscopy (dOPM) Biomed. 光学 快报，11，7204-7220 (2020) Philipp Zelger, Lisa Bodner, Martin Offterdinger, Lukas Velas, Gerhard J. Schütz, and Alexander Jesacher Three-dimensional single molecule localization close to the coverslip: a comparison of methods exploiting supercritical angle fluorescence Biomed. 光学 快报，12，802-822 (2021)
PC 建议
随着 ORCA-Fusion 的推出，用户现在能够以每秒 89 帧的速度将 500 万像素的图像流式传输到他们的计算机。通过使用 ORCA-Fusion PC 建议列出的指南，可以满足计算机对高数据率的建议。
软件
详细参数
| 类型编号 | C14440-20UP |
|---|---|
| 成像设备 | sCMOS |
| 有效像素数 | 2304(H)×2304(V) |
| 细胞大小 | 6.5 μm(H)×6.5 μm(V) |
| 有效面积 | 14.976 mm (H)×14.976 mm (V) |
| 最大阱容 | 15,000 个电子 *1 |
| 读出速度 | 快速扫描：11.22 ms（CoaXPress 为 89.1 帧/秒，USB 3.0 为 31.6 帧/秒）（2304 x 2304）*5 标准扫描：42.99 ms（23.2 帧/秒，带 CoaXPress 或 USB 3.0）（2304 x 2304）*5 超静音扫描：184.4 ms（5.4 帧/秒，带 CoaXPress 或 USB 3.0）（2304 x 2304）*5 |
| 读出噪声 | 快速扫描：1.4 个电子 rms *1 标准扫描：1.0 个电子 rms *1 超静音扫描：0.7 个电子 rms *1 |
| 曝光时间 | 快速扫描：17 μs 至 10 s（4.87 μs 步长） 标准扫描：65 μs 至 10 s（18.65 μs 步长） 超静音扫描：280 μs| 10 s（80.00 μs 步长） |
| 冷却方法 | 珀耳帖制冷 |
| 冷却温度 | 强制风冷：-5 ̊C（环境温度：+25 ̊C） 水冷：-5 ̊C（水温：+25 ̊C） 水冷（最大冷却）：低于 -15 ̊C *1，*3 |
| 暗电流 | 冷却温度 (-5 ̊C)：0.5 个电子/像素/秒 *1，*4 冷却温度 (-15 ̊C)：0.2 个电子/像素/秒 *1，*4 |
| 动态范围 | 21,400：1 *1，*2 |
| 外部触发模式 | 边缘、电平、同步读出、开始、全局复位边缘、全局复位电平 |
| 外部触发信号路由 | SMA |
| 触发延迟功能 | 0 s 至 10 s，步长为 1 μs |
| 触发输出 | 3 次可编程定时、全局曝光定时、触发就绪、低、高 |
| 外部信号输出路由 | SMA |
| 接口 | CoaXPress（双 CXP-6）和 USB 3.0 *6 |
| AD 转换器 | 16 位/12 位/8 位 |
| 透镜接口 | C 型接口 |
| 电源 | 约 150 VA *7 |
| 操作环境温度 | 0 ̊C 至 + 40 ̊C |
| 操作环境湿度 | 30% 至 80%，无冷凝 |
*1 典型值
*2 根据最大阱容和读出噪声的比率计算
*3 水温为 +20 ̊C，环境温度为 +20 ̊C
*4 暗电流取决于冷却温度
*5 有效至 4 位，四舍五入至第 5 位
*6 兼容 USB 3.1 Gen 1
*7 AC 240 V 时的值（约 70 VA，交流 100 V）
光谱灵敏度特性
尺寸
说明手册
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